Engineering a customizable antibacterial T6SS-based platform in Vibrio natriegens

Biswanath Jana, Kinga Keppel, Dor Salomon

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial pathogens are a major risk to human, animal, and plant health. To counteract the spread of antibiotic resistance, alternative antibacterial strategies are urgently needed. Here, we construct a proof-of-concept customizable, modular, and inducible antibacterial toxin delivery platform. By engineering a type VI secretion system (T6SS) that is controlled by an externally induced on/off switch, we transform the safe bacterium, Vibrio natriegens, into an effective antibacterial weapon. Furthermore, we demonstrate that the delivered effector repertoire, and thus the toxicity range of this platform, can be easily manipulated and tested. We believe that this platform can serve as a foundation for novel antibacterial bio-treatments, as well as a unique tool to study antibacterial toxins.

Original languageEnglish
Article numbere53681
JournalEMBO Reports
Volume22
Issue number11
DOIs
StatePublished - 4 Nov 2021

Keywords

  • Bacterial competition
  • effector
  • secretion system
  • synthetic biology
  • toxin

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