Engineered vascularized muscle flap

Dana Egozi, Yulia Shandalov, Alina Freiman, Dekel Rosenfeld, David Ben-Shimol, Shulamit Levenberg

Research output: Contribution to journalArticlepeer-review

Abstract

One of the main factors limiting the thickness of a tissue construct and its consequential viability and applicability in vivo, is the control of oxygen supply to the cell microenvironment, as passive diffusion is limited to a very thin layer. Although various materials have been described to restore the integrity of full-thickness defects of the abdominal wall, no material has yet proved to be optimal, due to low graft vascularization, tissue rejection, infection, or inadequate mechanical properties. This protocol describes a means of engineering a fully vascularized flap, with a thickness relevant for muscle tissue reconstruction. Cell-embedded poly L-lactic acid/poly lactic-co-glycolic acid constructs are implanted around the mouse femoral artery and vein and maintained in vivo for a period of one or two weeks. The vascularized graft is then transferred as a flap towards a full thickness defect made in the abdomen. This technique replaces the need for autologous tissue sacrifications and may enable the use of in vitro engineered vascularized flaps in many surgical applications.

Original languageEnglish
Article numbere52984
JournalJournal of Visualized Experiments
Volume2016
Issue number107
DOIs
StatePublished - 11 Jan 2016
Externally publishedYes

Keywords

  • Bioengineering
  • Flap
  • Issue 107
  • Mouse model
  • Muscle
  • Scaffold
  • Tissue engineering
  • Vascularization
  • Vascularized tissue

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