TY - JOUR
T1 - Energetics and chronology of phototransients in the light response of the purple membrane of Halobacterium halobium
AU - Sherman, Warren V.
AU - Korenstein, Rafi
AU - Caplan, S. Roy
N1 - Funding Information:
This project was supported by grants from the U.S.-Israel Binational Science Foundation (BSF), Jerusalem, Israel, and the West German Ministry of Science and Technology. One of us (W.V.S.) wishes to thank the European Molecular Biology Organization for a Fellowship.
PY - 1976/6/8
Y1 - 1976/6/8
N2 - Arrhenius parameters for formation and decay of phototransients in suspensions of purple membrane fragments in H2O and 2H2O have been determined in the temperature range 0-60 °C. Kinetic isotope effects are found which show that proton transfer steps are involved in both formation and decay of the two longest-lived transients absorbing at 410 nm and 660 nm, respectively. The results also suggest that these transients do not occupy a single pathway in the spontaneous deexcitation of bacteriorhodopsin within the purple membrane. Purple membrane undergoes a phase transition at 25-30 °C in both H2O and 2H2O.
AB - Arrhenius parameters for formation and decay of phototransients in suspensions of purple membrane fragments in H2O and 2H2O have been determined in the temperature range 0-60 °C. Kinetic isotope effects are found which show that proton transfer steps are involved in both formation and decay of the two longest-lived transients absorbing at 410 nm and 660 nm, respectively. The results also suggest that these transients do not occupy a single pathway in the spontaneous deexcitation of bacteriorhodopsin within the purple membrane. Purple membrane undergoes a phase transition at 25-30 °C in both H2O and 2H2O.
UR - http://www.scopus.com/inward/record.url?scp=0017083046&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(76)90021-9
DO - 10.1016/0005-2728(76)90021-9
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AN - SCOPUS:0017083046
SN - 0005-2728
VL - 430
SP - 454
EP - 458
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 3
ER -