Energetics and chronology of phototransients in the light response of the purple membrane of Halobacterium halobium

Warren V. Sherman*, Rafi Korenstein, S. Roy Caplan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Arrhenius parameters for formation and decay of phototransients in suspensions of purple membrane fragments in H2O and 2H2O have been determined in the temperature range 0-60 °C. Kinetic isotope effects are found which show that proton transfer steps are involved in both formation and decay of the two longest-lived transients absorbing at 410 nm and 660 nm, respectively. The results also suggest that these transients do not occupy a single pathway in the spontaneous deexcitation of bacteriorhodopsin within the purple membrane. Purple membrane undergoes a phase transition at 25-30 °C in both H2O and 2H2O.

Original languageEnglish
Pages (from-to)454-458
Number of pages5
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume430
Issue number3
DOIs
StatePublished - 8 Jun 1976
Externally publishedYes

Funding

FundersFunder number
U.S.-Israel Binational Science Foundation
European Molecular Biology Organization
United States-Israel Binational Science Foundation
Ministry of Science and Technology, Israel

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