TY - JOUR
T1 - Endogenous RNA cleavages at the ribosomal SRL site likely reflect miRNA (miR) mediated translational suppression
AU - Pichinuk, Edward
AU - Broday, Limor
AU - Wreschner, Daniel H.
N1 - Funding Information:
This research was funded in part by grants from the Israel Cancer Association (20112024) and Israel Science Foundation (1167/10).
PY - 2011/11/4
Y1 - 2011/11/4
N2 - We previously suggested a mechanism whereby the RNA induced silencing complex (RISC) brings about a specific cleavage at the sarcin-ricin loop (SRL) of 28S ribosomal RNA thereby eliciting translational suppression. Here we experimentally show that endogenous cleavages take place at the SRL site, in both mammalian cells and in Caenorhabditis elegans. Furthermore we demonstrate that bulged and looped-out residues present in the imperfect miRNA-[mRNA target site] duplexes, are complementary to the SRL site. These results support, and are compatible with, our described mechanism whereby microRNAs mediate cleavage of the highly conserved 28S rRNA sarcin/ricin loop leading to translational suppression.
AB - We previously suggested a mechanism whereby the RNA induced silencing complex (RISC) brings about a specific cleavage at the sarcin-ricin loop (SRL) of 28S ribosomal RNA thereby eliciting translational suppression. Here we experimentally show that endogenous cleavages take place at the SRL site, in both mammalian cells and in Caenorhabditis elegans. Furthermore we demonstrate that bulged and looped-out residues present in the imperfect miRNA-[mRNA target site] duplexes, are complementary to the SRL site. These results support, and are compatible with, our described mechanism whereby microRNAs mediate cleavage of the highly conserved 28S rRNA sarcin/ricin loop leading to translational suppression.
KW - Alpha-sarcin
KW - MiRNA
KW - RRNA cleavage
KW - SRL
KW - Translational suppression
UR - http://www.scopus.com/inward/record.url?scp=80555122775&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2011.09.140
DO - 10.1016/j.bbrc.2011.09.140
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AN - SCOPUS:80555122775
SN - 0006-291X
VL - 414
SP - 706
EP - 711
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -