TY - JOUR
T1 - Emergence and transmission of arbovirus evolutionary intermediates with epidemic potential
AU - Stapleford, Kenneth A.
AU - Coffey, Lark L.
AU - Lay, Sreyrath
AU - Bordería, Antonio V.
AU - Duong, Veasna
AU - Isakov, Ofer
AU - Rozen-Gagnon, Kathryn
AU - Arias-Goeta, Camilo
AU - Blanc, Hervé
AU - Beaucourt, Stéphanie
AU - Haliloǧlu, Türkan
AU - Schmitt, Christine
AU - Bonne, Isabelle
AU - Ben-Tal, Nir
AU - Shomron, Noam
AU - Failloux, Anna Bella
AU - Buchy, Philippe
AU - Vignuzzi, Marco
N1 - Funding Information:
This work was supported by the European Research Council (ERC Starting Grant No. 242719), by the Bill and Melinda Gates Foundation Grand Challenges Exploration Initiative, the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” (grant ANR-10-LABX-62-IBEID), and the Israel-France High Council for Science and Technology Research “Complexity in Biology” program. Salary for L.L.C. and K.A.S. was provided by the Region of Ile-de-France DIM program on Infectious, Parasitic or Nosocomial Emerging Diseases and the Philippe Foundation. Salary for A.V.B. was supported by the French National grant ANR-09-JCJC-0118-1. Salary for C.A.-G. was supported by the French Ministry of Superior Education and Research. N.B.-T. and T.H. were supported by NATO traveling grant CBP.MD.CLG 984340. We are grateful to Ngan Chantha, Huy Rekol, and the team of the National Malaria Centre in Cambodia for providing clinical specimens. We thank Christophe Paupy (IRD), Louis Lambrechts, and Paul Reiter (IP) for providing some mosquito strains.
PY - 2014/6/11
Y1 - 2014/6/11
N2 - The high replication and mutation rates of RNA viruses can result in the emergence of new epidemic variants. Thus, the ability to follow host-specific evolutionary trajectories of viruses is essential to predict and prevent epidemics. By studying the spatial and temporal evolution of chikungunya virus during natural transmission between mosquitoes and mammals, we have identified viral evolutionary intermediates prior to emergence. Analysis of virus populations at anatomical barriers revealed that the mosquito midgut and salivary gland pose population bottlenecks. By focusing on virus subpopulations in the saliva of multiple mosquito strains, we recapitulated the emergence of a recent epidemic strain of chikungunya and identified E1 glycoprotein mutations with potential to emerge in the future. These mutations confer fitness advantages in mosquito and mammalian hosts by altering virion stability and fusogenic activity. Thus, virus evolutionary trajectories can be predicted and studied in the short term before new variants displace currently circulating strains.
AB - The high replication and mutation rates of RNA viruses can result in the emergence of new epidemic variants. Thus, the ability to follow host-specific evolutionary trajectories of viruses is essential to predict and prevent epidemics. By studying the spatial and temporal evolution of chikungunya virus during natural transmission between mosquitoes and mammals, we have identified viral evolutionary intermediates prior to emergence. Analysis of virus populations at anatomical barriers revealed that the mosquito midgut and salivary gland pose population bottlenecks. By focusing on virus subpopulations in the saliva of multiple mosquito strains, we recapitulated the emergence of a recent epidemic strain of chikungunya and identified E1 glycoprotein mutations with potential to emerge in the future. These mutations confer fitness advantages in mosquito and mammalian hosts by altering virion stability and fusogenic activity. Thus, virus evolutionary trajectories can be predicted and studied in the short term before new variants displace currently circulating strains.
UR - http://www.scopus.com/inward/record.url?scp=84902440512&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2014.05.008
DO - 10.1016/j.chom.2014.05.008
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:84902440512
SN - 1931-3128
VL - 15
SP - 706
EP - 716
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 6
ER -