Abstract
Preimplantation genetic screening (PGS) has been one of the most promising advancements in reproductive medicine. Current PGS practice employs comprehensive chromosomal screening. This progressive technique, particularly next-generation sequencing (NGS), has revealed the phenomenon of embryonic mosaic aneuploidy, the presence of cell lineages with different chromosomal constitutions. Two Web-based PGS surveys conducted about reproductive medicine practitioners assessed, among other topics, attitudes and opinions regarding mosaicism in PGS. Published survey results highlighted a number of distinct differences between practitioners in facilities that performed PGS and those that did not. There were also gaps in consensus on the objectives of PGS, how often mosaicism was encountered and reported, whether NGS was required to detect mosaicism, and what the maximum aneuploid cell percentage should be employed for performing embryo transfers. These discrepancies ultimately lead to differences in opinion regarding whether PGS should be performed if mosaicism decision-making guidelines are not yet available.
Original language | English |
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Title of host publication | Human Embryos and Preimplantation Genetic Technologies |
Subtitle of host publication | Ethical, Social, and Public Policy Aspects |
Publisher | Elsevier |
Pages | 35-42 |
Number of pages | 8 |
ISBN (Electronic) | 9780128164686 |
DOIs | |
State | Published - 1 Jan 2019 |
Keywords
- Assisted reproductive technology
- Comprehensive chromosomal screening
- Mosaicism
- Next-generation sequencing
- Preimplantation genetic screening