ELISA of anti-endomysial antibodies in the diagnosis of celiac disease: Comparison with immunofluorescence assay of anti-endomysial antibodies and tissue transglutaminase antibodies

Raanan Shamir*, Rami Eliakim, Nitza Lahat, Esther Sobel, Aaron Lerner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Celiac disease is common in both children and adults. Small intestinal biopsy is mandatory for establishing a diagnosis. Anti-endomysial antibodies, detected by immunofluorescence, have a sensitivity and specificity close to 100% in the diagnosis of CD. Recently, tissue transglutaminase has been identified as the target autoantigen of antibodies against endomysium, and TTG antibodies are comparable to EMA-IMF in the diagnosis of CD. Objective: To evaluate a new enzyme-linked immunosorbent assay kit for EMA, compared to EMA-IMF and TTG antibodies in the diagnosis of CD. Methods: Our study population included all subjects with positive EMA-IMF who underwent intestinal biopsy (n=21). From the same sera, TTG antibodies and EMA-ELISA were determined, and all antibody results were compared to the biopsy findings. Results: EMA-IMF was able to predict biopsy findings of CD in 19 of 21 cases (90.5%). When patients with biopsy findings compatible with CD and positive EMA-IMF (n = 19) were tested for EMA-ELISA and TTG antibodies, 18 of the 19 were positive for both EMA-ELISA and TTG antibodies. A significant correlation was found between EMA-ELISA and TTG antibody titers (r = 0.74, P < 0.001). Conclusions: Our study demonstrates that EMA-ELISA is comparable to TTG antibodies in the diagnosis of CD, and supports the use of EMA-ELISA as a serologic marker for this disease.

Original languageEnglish
Pages (from-to)594-596
Number of pages3
JournalIsrael Medical Association Journal
Volume4
Issue number8
StatePublished - 2002
Externally publishedYes

Keywords

  • Anti-endomysial antibodies
  • Celiac disease
  • Enzyme-linked immunosorbent assay
  • Immunofluorescence
  • Tissue transglutaminase antibodies

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