Electrospun Fibrous PVDF-TrFe Scaffolds for Cardiac Tissue Engineering, Differentiation, and Maturation

Nofar Adadi, Moran Yadid, Idan Gal, Masha Asulin, Ron Feiner, Reuven Edri, Tal Dvir*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Cardiac tissue engineering aims to create cardiac tissue constructs that recapitulate the structure and function of the native heart. This approach has been widely used for creating myocardial implants for regenerative medicine, and more recently, for developing in vitro cardiotoxicity screening assays. However, once the engineered myocardial tissues are implanted or subjected to pharmacological stimuli, their performance should be monitored. Currently, there is no biomaterial that promotes functional tissues assembly while providing real-time information about their function, in situ. In this study, the piezoelectric phenomenon is sought to be exploited, to measure the contractions generated by engineered cardiac tissues. A poly-(vinylidene fluoride) (PVDF)-based electrospun fiber scaffold is developed, and it is hypothesized that the contractions of cardiomyocytes in the scaffold will induce mechanical deformations, which will result in measurable electric voltage. The PVDF scaffolds are characterized and optimized for supporting formation of aligned, functional, cardiac tissues. The scaffolds' function is then validated as sensors for tissue contraction and it is demonstrated that they can sense contractions of tissues constructed from as few as 5 × 105 cardiomyocytes. Furthermore, it is demonstrated that human induced pluripotent stem cells can be directly seeded and differentiated to cardiomyocytes, and then mature over the course of 40 days on the PVDF fiber scaffolds.

Original languageEnglish
Article number1900820
JournalAdvanced Materials Technologies
Volume5
Issue number3
DOIs
StatePublished - 1 Mar 2020

Funding

FundersFunder number
Ben Gurion University
Slezak Foundation
Horizon 2020 Framework Programme637943
European Research Council
Ministry of Science, Technology and Space3‐14412
Israel Science Foundation700/13

    Keywords

    • cardiac differentiation
    • engineered tissues
    • heart patches
    • iPSCs
    • piezoelectric scaffolds

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