@inproceedings{321e7079c1da4960aae6eac96fc53b99,
title = "Electroporation delivered protein biosensors for study of molecular activity on microfluidic platform",
abstract = "Fluorescence resonance energy transfer (FRET)-based protein biosensors report intracellular molecular activities with high spatiotemporal resolution via optical signal. A critical limit for these biosensors has been the requirement of genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation on microfluidic platform, we achieve sufficient concentration of the biosensor inside cells and observe strong FRET signal that is indicative of molecular activity. Our method does not require delivery and expression of the biosensor plasmid and thus is suitable for samples size down to ∼100 cells.",
keywords = "Electroporation, FRET, Microfluidic, Protein biosensor",
author = "Chen Sun and Mingxing Ouyang and Zhenning Cao and Sai Ma and Yingxiao Wang and Chang Lu",
note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
year = "2014",
language = "אנגלית",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "2014--2016",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
}