Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

Chen Sun; Mingxing Ouyang; Zhenning Cao; Sai Ma; Hamzeh Alqublan; Nammalwar Sriranganathan; Yingxiao Wang; Chang Lu

doi:10.1039/c4cc04730c

Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

Chen Sun, Mingxing Ouyang, Zhenning Cao, Sai Ma, Hamzeh Alqublan, Nammalwar Sriranganathan, Yingxiao Wang, Chang Lu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ∼100 cells with high spatiotemporal resolution.

Original language	English
Pages (from-to)	11536-11539
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	78
DOIs	https://doi.org/10.1039/c4cc04730c
State	Published - 4 Sep 2014
Externally published	Yes

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abstract = "Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ∼100 cells with high spatiotemporal resolution.",

author = "Chen Sun and Mingxing Ouyang and Zhenning Cao and Sai Ma and Hamzeh Alqublan and Nammalwar Sriranganathan and Yingxiao Wang and Chang Lu",

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AU - Ouyang, Mingxing

AU - Cao, Zhenning

AU - Ma, Sai

AU - Alqublan, Hamzeh

AU - Sriranganathan, Nammalwar

AU - Wang, Yingxiao

AU - Lu, Chang

PY - 2014/9/4

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N2 - Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ∼100 cells with high spatiotemporal resolution.

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JO - Chemical Communications

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Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

Abstract

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