Electroporation-Based Biopsy Treatment Planning with Numerical Models and Tissue Phantoms

Batel Gabay, Klimentiy Levkov, Ariel Berl, Julia Wise, Ofir Shir-az, Edward Vitkin, Gintautas Saulis, Avshalom Shalom, Alexander Golberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Molecular sampling with vacuum-assisted tissue electroporation is a novel, minimally invasive method for molecular profiling of solid lesions. In this paper, we report on the design of the battery-powered pulsed electric field generator and electrode configuration for an electroporation-based molecular sampling device for skin cancer diagnostics. Using numerical models of skin electroporation corroborated by the potato tissue phantom model, we show that the electroporated tissue volume, which is the maximum volume for biomarker sampling, strongly depends on the electrode’s geometry, needle electrode skin penetration depths, and the applied pulsed electric field protocol. In addition, using excised human basal cell carcinoma (BCC) tissues, we show that diffusion of proteins out of human BCC tissues into water strongly depends on the strength of the applied electric field and on the time after the field application. The developed numerical simulations, confirmed by experiments in potato tissue phantoms and excised human cancer lesions, provide essential tools for the development of electroporation-based molecular markers sampling devices for personalized skin cancer diagnostics.

Original languageEnglish
Pages (from-to)71-88
Number of pages18
JournalAnnals of Biomedical Engineering
Issue number1
StatePublished - Jan 2024


FundersFunder number
Tel Aviv University
Ministry of Science and Technology, Israel


    • Dermatology
    • Diagnostics
    • Finite elements modeling
    • Medical devices
    • Molecular pathology
    • Skin cancer
    • Skin electroporation


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