Electroporation and Agrobacterium-mediated spore transformation.

Anna Minz, Amir Sharon

Research output: Contribution to journalArticlepeer-review

Abstract

Genetic transformation is a key technology in modern fungal research. Most commonly, protoplasts are transformed using the polyethylene glycol-mediated transformation protocols. Because protoplasts are generated by treatment of mycelia with a crude enzyme preparation, the results tend to be inconsistent. Furthermore, some species cannot be transformed by this method. Electroporation (EP) and Agrobacterium tumefaciens-mediated transformation (AMT) are two alternative methods. These methods allow the transformation of spores or mycelia, they are simple to perform and provide consistent results. In this chapter, we describe EP and AMT protocols for the fungus Colletotrichum gloeosporioides f. sp. aeschynomene (C. gloeosporioides). These protocols can be used as baseline for the calibration of similar transformation protocols in other species.

Original languageEnglish
Pages (from-to)21-32
Number of pages12
JournalMethods in Molecular Biology
Volume638
DOIs
StatePublished - 2010

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