TY - JOUR
T1 - Electroporation and Agrobacterium-mediated spore transformation.
AU - Minz, Anna
AU - Sharon, Amir
PY - 2010
Y1 - 2010
N2 - Genetic transformation is a key technology in modern fungal research. Most commonly, protoplasts are transformed using the polyethylene glycol-mediated transformation protocols. Because protoplasts are generated by treatment of mycelia with a crude enzyme preparation, the results tend to be inconsistent. Furthermore, some species cannot be transformed by this method. Electroporation (EP) and Agrobacterium tumefaciens-mediated transformation (AMT) are two alternative methods. These methods allow the transformation of spores or mycelia, they are simple to perform and provide consistent results. In this chapter, we describe EP and AMT protocols for the fungus Colletotrichum gloeosporioides f. sp. aeschynomene (C. gloeosporioides). These protocols can be used as baseline for the calibration of similar transformation protocols in other species.
AB - Genetic transformation is a key technology in modern fungal research. Most commonly, protoplasts are transformed using the polyethylene glycol-mediated transformation protocols. Because protoplasts are generated by treatment of mycelia with a crude enzyme preparation, the results tend to be inconsistent. Furthermore, some species cannot be transformed by this method. Electroporation (EP) and Agrobacterium tumefaciens-mediated transformation (AMT) are two alternative methods. These methods allow the transformation of spores or mycelia, they are simple to perform and provide consistent results. In this chapter, we describe EP and AMT protocols for the fungus Colletotrichum gloeosporioides f. sp. aeschynomene (C. gloeosporioides). These protocols can be used as baseline for the calibration of similar transformation protocols in other species.
UR - http://www.scopus.com/inward/record.url?scp=77953631332&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-611-5_2
DO - 10.1007/978-1-60761-611-5_2
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AN - SCOPUS:77953631332
SN - 1064-3745
VL - 638
SP - 21
EP - 32
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -