TY - JOUR
T1 - Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter
AU - Gershoni, Jonathan M.
AU - Palade, George E.
N1 - Funding Information:
We thank Dr. Pietro DeCamilli, Ms. Penny Miller, and Dr. Carl Roman0 for providing samples of bovine brain homogenates and anti-protein I; Dr. Ira Mellman for useful advice “with protein radioiodination; Mrs. Cindy Davis for typing the manuscript; and Dr. Zaid Smith for sustained critical discussion. This work was supported by NIH Grant GM 27303 and by a Chaim Weizmann Postdoctoral Fellowship (J. M. G).
PY - 1982/8
Y1 - 1982/8
N2 - Zeta-bind, a positively charged nylon membrane, was tested as an immobilizing matrix for the electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels. It was found that Zeta-bind has a considerably greater capacity than does nitrocellulose for protein binding. Because of this property, more efficient elution of proteins from gels can be used (by omitting methanol from transfer buffers). The procedure described is more amenable to quantitation than usual nitrocellulose-based transfer. Antibody or lectin overlay techniques are also more sensitive on Zeta-bind than on nitrocellulose.
AB - Zeta-bind, a positively charged nylon membrane, was tested as an immobilizing matrix for the electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels. It was found that Zeta-bind has a considerably greater capacity than does nitrocellulose for protein binding. Because of this property, more efficient elution of proteins from gels can be used (by omitting methanol from transfer buffers). The procedure described is more amenable to quantitation than usual nitrocellulose-based transfer. Antibody or lectin overlay techniques are also more sensitive on Zeta-bind than on nitrocellulose.
UR - http://www.scopus.com/inward/record.url?scp=0020170968&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(82)90056-2
DO - 10.1016/0003-2697(82)90056-2
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AN - SCOPUS:0020170968
SN - 0003-2697
VL - 124
SP - 396
EP - 405
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -