Electrophoretic characterization of human phosphoribosylpyrophosphate synthetase

P. Boer*, O. Sperling, A. De Vries

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A method has been developed for the electrophoretic separation of 5-phosphoribosyl-1-pyrophosphate synthetase on cellulose acetate. The human enzyme in extracts of various tissues appears as a narrow symmetrical peak, indicating a single genotype. Similar results were obtained with rabbit and rat tissues. A mutant erythrocyte 5-phosphoribosyl-1-pyrophosphate synthetase from two siblings having gout associated with excessive purine production was found to have normal electrophoretic mobility.

Original languageEnglish
Pages (from-to)175-179
Number of pages5
JournalBiochemical Medicine
Issue number2
StatePublished - Jun 1974


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