Electron microscopic evidence for splicing of SV40 late mRNAs

Susan Bratosin; Mia Horowitz; Orgad Laub; Yosef Aloni

doi:10.1016/0092-8674(78)90228-3

Electron microscopic evidence for splicing of SV40 late mRNAs

Susan Bratosin^*, Mia Horowitz, Orgad Laub, Yosef Aloni

^*Corresponding author for this work

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Poly(A)-containing SV40 cytoplasmic RNA was hybridized with linear double-stranded SV40 DNA and formed RNA displacement loops (R loops). The structures visualized in the electron microscope are consistent with the conclusion that the leader sequences at the 5′ ends of the 16S and 19S late mRNAs are not coded immediately adjacent to the main portions of the mRNAs. These data are consistent with either segmentation of the leaders or heterogeneity of their lengths. Measurements carried out on the R loop structures have provided the locations, on the physical map of SV40 DNA, for the bodies and leaders of the 16S and 19S late mRNAs, and the lengths of the bodies, leaders and the corresponding intervening DNA sequences.

Original language	English
Pages (from-to)	783-790
Number of pages	8
Journal	Cell
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(78)90228-3
State	Published - Apr 1978
Externally published	Yes

Funding

Funders	Funder number
U.S. Public Health Service

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10.1016/0092-8674(78)90228-3

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title = "Electron microscopic evidence for splicing of SV40 late mRNAs",

abstract = "Poly(A)-containing SV40 cytoplasmic RNA was hybridized with linear double-stranded SV40 DNA and formed RNA displacement loops (R loops). The structures visualized in the electron microscope are consistent with the conclusion that the leader sequences at the 5′ ends of the 16S and 19S late mRNAs are not coded immediately adjacent to the main portions of the mRNAs. These data are consistent with either segmentation of the leaders or heterogeneity of their lengths. Measurements carried out on the R loop structures have provided the locations, on the physical map of SV40 DNA, for the bodies and leaders of the 16S and 19S late mRNAs, and the lengths of the bodies, leaders and the corresponding intervening DNA sequences.",

author = "Susan Bratosin and Mia Horowitz and Orgad Laub and Yosef Aloni",

note = "Funding Information: This research was supported by a USPHS research grant.",

year = "1978",

month = apr,

doi = "10.1016/0092-8674(78)90228-3",

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T1 - Electron microscopic evidence for splicing of SV40 late mRNAs

AU - Bratosin, Susan

AU - Horowitz, Mia

AU - Laub, Orgad

AU - Aloni, Yosef

N1 - Funding Information: This research was supported by a USPHS research grant.

PY - 1978/4

Y1 - 1978/4

N2 - Poly(A)-containing SV40 cytoplasmic RNA was hybridized with linear double-stranded SV40 DNA and formed RNA displacement loops (R loops). The structures visualized in the electron microscope are consistent with the conclusion that the leader sequences at the 5′ ends of the 16S and 19S late mRNAs are not coded immediately adjacent to the main portions of the mRNAs. These data are consistent with either segmentation of the leaders or heterogeneity of their lengths. Measurements carried out on the R loop structures have provided the locations, on the physical map of SV40 DNA, for the bodies and leaders of the 16S and 19S late mRNAs, and the lengths of the bodies, leaders and the corresponding intervening DNA sequences.

AB - Poly(A)-containing SV40 cytoplasmic RNA was hybridized with linear double-stranded SV40 DNA and formed RNA displacement loops (R loops). The structures visualized in the electron microscope are consistent with the conclusion that the leader sequences at the 5′ ends of the 16S and 19S late mRNAs are not coded immediately adjacent to the main portions of the mRNAs. These data are consistent with either segmentation of the leaders or heterogeneity of their lengths. Measurements carried out on the R loop structures have provided the locations, on the physical map of SV40 DNA, for the bodies and leaders of the 16S and 19S late mRNAs, and the lengths of the bodies, leaders and the corresponding intervening DNA sequences.

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JO - Cell

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Electron microscopic evidence for splicing of SV40 late mRNAs

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