Electron Ionization Mass Spectrometry for Both Liquid and Gas Chromatography in One System without the Need for Hardware Adjustments

A new instrument that bridges the gap between gas chromatography (GC) and liquid chromatography (LC) mass spectrometry (MS) was developed. In this instrument GC-MS and electron ionization LC-MS were combined in one MS system with method based mode changing. The LC pneumatic spray formation interface to MS was mounted on top of an otherwise unused GC detector slot and was connected with a flow restriction capillary to the MS through the GC oven and into the MS transfer line, parallel to the GC capillary column. The LC output mobile phase flow is directed into a spray formation and vaporization chamber. The pneumatic spray results in fine spray droplets that are thermally vaporized at a pressure equal to or greater than ambient. A portion of the vaporized mixture is directed into the heated flow restriction capillary that connects the spray formation and vaporization chamber into the electron ionization (EI) ion source, while most of the vaporized spray is released to the atmosphere. The combined GC-MS and LC-MS system can work either with standard EI or with cold EI via interfacing the flow restriction capillary into a supersonic nozzle forming a supersonic molecular beam of a vibrationally cold sample compound. We found that EI-LC-MS with cold EI has many benefits when compared with standard EI. The EI-LC-MS interface can also serve for flow injection analysis. The performance of the combined system is demonstrated in the analysis of a few sample mixtures by both GC-MS and LC-MS analysis, sequentially without hardware adjustments.