Electrogenic and selective transport of biogenic amines across lipid bilayer membranes mediated by a neutral ionophore (AS701)

Rimona Margalit; Amira Zeevi; Abraham Shanzer

doi:10.1016/0005-2736(84)90291-8

Electrogenic and selective transport of biogenic amines across lipid bilayer membranes mediated by a neutral ionophore (AS701)

Rimona Margalit^*, Amira Zeevi, Abraham Shanzer

^*Corresponding author for this work

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The neutral noncyclic imide and ether containing ionophore (AS701), a selective carrier for Li⁺ among alkali cations, was found to be capable of mediating the transport of NH₄⁺ and of biogenic amines (catechols and indoles) across lipid bilayer membranes also. Ionophore-mediated electrical properties of planar lipid bilayers were studied under experimental conditions where the positively-charged amine species was dominant. The ionophore was found to act as a selective carrier of the biogenic amines, mediating their electrogenic transport across the membrane, forming 2:1 carrier-amine permeant complexes, carrying a net-charge of +1. Selectively among the amines corresponding to the following sequence: tryptamine (35) > Li⁺ (1) > serotonin (0.60) > dopamine (0.19) > norepinephrine (0.13) > epinephrine (0.05) > NH₄⁺ (0.05). The molecular factors involved in determining these selectivities are assessed.

Original language	English
Pages (from-to)	193-198
Number of pages	6
Journal	BBA - Biomembranes
Volume	774
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(84)90291-8
State	Published - 25 Jul 1984

Keywords

(Lipid bilayer)
Biogenic amine
Electrogenic transport
Ionophore AS701

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10.1016/0005-2736(84)90291-8

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title = "Electrogenic and selective transport of biogenic amines across lipid bilayer membranes mediated by a neutral ionophore (AS701)",

abstract = "The neutral noncyclic imide and ether containing ionophore (AS701), a selective carrier for Li+ among alkali cations, was found to be capable of mediating the transport of NH4+ and of biogenic amines (catechols and indoles) across lipid bilayer membranes also. Ionophore-mediated electrical properties of planar lipid bilayers were studied under experimental conditions where the positively-charged amine species was dominant. The ionophore was found to act as a selective carrier of the biogenic amines, mediating their electrogenic transport across the membrane, forming 2:1 carrier-amine permeant complexes, carrying a net-charge of +1. Selectively among the amines corresponding to the following sequence: tryptamine (35) > Li+ (1) > serotonin (0.60) > dopamine (0.19) > norepinephrine (0.13) > epinephrine (0.05) > NH4+ (0.05). The molecular factors involved in determining these selectivities are assessed.",

keywords = "(Lipid bilayer), Biogenic amine, Electrogenic transport, Ionophore AS701",

author = "Rimona Margalit and Amira Zeevi and Abraham Shanzer",

year = "1984",

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doi = "10.1016/0005-2736(84)90291-8",

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T1 - Electrogenic and selective transport of biogenic amines across lipid bilayer membranes mediated by a neutral ionophore (AS701)

AU - Margalit, Rimona

AU - Zeevi, Amira

AU - Shanzer, Abraham

PY - 1984/7/25

Y1 - 1984/7/25

N2 - The neutral noncyclic imide and ether containing ionophore (AS701), a selective carrier for Li+ among alkali cations, was found to be capable of mediating the transport of NH4+ and of biogenic amines (catechols and indoles) across lipid bilayer membranes also. Ionophore-mediated electrical properties of planar lipid bilayers were studied under experimental conditions where the positively-charged amine species was dominant. The ionophore was found to act as a selective carrier of the biogenic amines, mediating their electrogenic transport across the membrane, forming 2:1 carrier-amine permeant complexes, carrying a net-charge of +1. Selectively among the amines corresponding to the following sequence: tryptamine (35) > Li+ (1) > serotonin (0.60) > dopamine (0.19) > norepinephrine (0.13) > epinephrine (0.05) > NH4+ (0.05). The molecular factors involved in determining these selectivities are assessed.

AB - The neutral noncyclic imide and ether containing ionophore (AS701), a selective carrier for Li+ among alkali cations, was found to be capable of mediating the transport of NH4+ and of biogenic amines (catechols and indoles) across lipid bilayer membranes also. Ionophore-mediated electrical properties of planar lipid bilayers were studied under experimental conditions where the positively-charged amine species was dominant. The ionophore was found to act as a selective carrier of the biogenic amines, mediating their electrogenic transport across the membrane, forming 2:1 carrier-amine permeant complexes, carrying a net-charge of +1. Selectively among the amines corresponding to the following sequence: tryptamine (35) > Li+ (1) > serotonin (0.60) > dopamine (0.19) > norepinephrine (0.13) > epinephrine (0.05) > NH4+ (0.05). The molecular factors involved in determining these selectivities are assessed.

KW - (Lipid bilayer)

KW - Biogenic amine

KW - Electrogenic transport

KW - Ionophore AS701

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SN - 0005-2736

VL - 774

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JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 2

ER -

Electrogenic and selective transport of biogenic amines across lipid bilayer membranes mediated by a neutral ionophore (AS701)

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