TY - JOUR
T1 - Electrogenic and selective transport of biogenic amines across lipid bilayer membranes mediated by a neutral ionophore (AS701)
AU - Margalit, Rimona
AU - Zeevi, Amira
AU - Shanzer, Abraham
PY - 1984/7/25
Y1 - 1984/7/25
N2 - The neutral noncyclic imide and ether containing ionophore (AS701), a selective carrier for Li+ among alkali cations, was found to be capable of mediating the transport of NH4+ and of biogenic amines (catechols and indoles) across lipid bilayer membranes also. Ionophore-mediated electrical properties of planar lipid bilayers were studied under experimental conditions where the positively-charged amine species was dominant. The ionophore was found to act as a selective carrier of the biogenic amines, mediating their electrogenic transport across the membrane, forming 2:1 carrier-amine permeant complexes, carrying a net-charge of +1. Selectively among the amines corresponding to the following sequence: tryptamine (35) > Li+ (1) > serotonin (0.60) > dopamine (0.19) > norepinephrine (0.13) > epinephrine (0.05) > NH4+ (0.05). The molecular factors involved in determining these selectivities are assessed.
AB - The neutral noncyclic imide and ether containing ionophore (AS701), a selective carrier for Li+ among alkali cations, was found to be capable of mediating the transport of NH4+ and of biogenic amines (catechols and indoles) across lipid bilayer membranes also. Ionophore-mediated electrical properties of planar lipid bilayers were studied under experimental conditions where the positively-charged amine species was dominant. The ionophore was found to act as a selective carrier of the biogenic amines, mediating their electrogenic transport across the membrane, forming 2:1 carrier-amine permeant complexes, carrying a net-charge of +1. Selectively among the amines corresponding to the following sequence: tryptamine (35) > Li+ (1) > serotonin (0.60) > dopamine (0.19) > norepinephrine (0.13) > epinephrine (0.05) > NH4+ (0.05). The molecular factors involved in determining these selectivities are assessed.
KW - (Lipid bilayer)
KW - Biogenic amine
KW - Electrogenic transport
KW - Ionophore AS701
UR - http://www.scopus.com/inward/record.url?scp=0021220682&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(84)90291-8
DO - 10.1016/0005-2736(84)90291-8
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AN - SCOPUS:0021220682
SN - 0005-2736
VL - 774
SP - 193
EP - 198
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 2
ER -