Electrogenic and selective transport of biogenic amines across lipid bilayer membranes mediated by a neutral ionophore (AS701)

Rimona Margalit*, Amira Zeevi, Abraham Shanzer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The neutral noncyclic imide and ether containing ionophore (AS701), a selective carrier for Li+ among alkali cations, was found to be capable of mediating the transport of NH4+ and of biogenic amines (catechols and indoles) across lipid bilayer membranes also. Ionophore-mediated electrical properties of planar lipid bilayers were studied under experimental conditions where the positively-charged amine species was dominant. The ionophore was found to act as a selective carrier of the biogenic amines, mediating their electrogenic transport across the membrane, forming 2:1 carrier-amine permeant complexes, carrying a net-charge of +1. Selectively among the amines corresponding to the following sequence: tryptamine (35) > Li+ (1) > serotonin (0.60) > dopamine (0.19) > norepinephrine (0.13) > epinephrine (0.05) > NH4+ (0.05). The molecular factors involved in determining these selectivities are assessed.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalBBA - Biomembranes
Volume774
Issue number2
DOIs
StatePublished - 25 Jul 1984

Keywords

  • (Lipid bilayer)
  • Biogenic amine
  • Electrogenic transport
  • Ionophore AS701

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