Electrochemical detection of protein-protein interactions using a yeast two hybrid: 17-β-Estradiol as a model

A. Schwartz-Mittelmann, T. Neufeld, D. Biran, J. Rishpon

Research output: Contribution to journalArticlepeer-review

Abstract

In this work we present a modified yeast two-hybrid bioassay for the highly sensitive detection of protein-protein interactions, based on the electrochemical monitoring of β-D-galactosidase reporter gene activity, using p-aminophenyl-β-D-galactopyranoside (PAPG) as a synthetic substrate. In a model system, the sensitive detection of 17-β-estradiol was achieved at concentrations as low as 10-11M (approx 2 pg/ml) by monitoring 17-β-estradiol receptor dimerization after exposure to 17-β-estradiol. The sensitivity of this system was higher than that of standard optical methods by three orders of magnitude.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalAnalytical Biochemistry
Volume317
Issue number1
DOIs
StatePublished - 1 Jun 2003

Keywords

  • Electrochemical
  • Estradiol
  • Protein-protein interaction
  • Yeast two hybrid

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