Electrochemical biochip characterization of the effect of formaldehyde on the activity of alkaline phosphatase

Carmit Porat-Ophir; Anton Belkin; Sefi Vernick; Vladimir Dergachev; Genrietta Freynd; Mikhail Katsnelson; Yosi Shacham-Diamand

doi:10.1149/2.006312eel

Electrochemical biochip characterization of the effect of formaldehyde on the activity of alkaline phosphatase

Carmit Porat-Ophir^*, Anton Belkin, Sefi Vernick, Vladimir Dergachev, Genrietta Freynd, Mikhail Katsnelson, Yosi Shacham-Diamand

^*Corresponding author for this work

School of Electrical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Alkaline phosphatase (ALP) is a colorectal cancer biomarker that can be electrochemically measured from biopsy samples. Since sampled tissues are usually preserved in formalin, it is crucial to know the effect of formaldehyde on ALP activity in the same biochip used for sampled tissue characterization. ALP was reacted with substrate 1-Naphtyl phosphate in a micro-chip with low frequency agitation and the product was oxidized at 300 mV. After exposure to formaldehyde, we identified a sharp initial drop in ALP activity followed by an exponential decrease with the exposure time with an effective time constant of ∼30 minutes.

Original language	English
Pages (from-to)	G8-G10
Journal	ECS Electrochemistry Letters
Volume	2
Issue number	12
DOIs	https://doi.org/10.1149/2.006312eel
State	Published - 2013

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10.1149/2.006312eel

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title = "Electrochemical biochip characterization of the effect of formaldehyde on the activity of alkaline phosphatase",

abstract = "Alkaline phosphatase (ALP) is a colorectal cancer biomarker that can be electrochemically measured from biopsy samples. Since sampled tissues are usually preserved in formalin, it is crucial to know the effect of formaldehyde on ALP activity in the same biochip used for sampled tissue characterization. ALP was reacted with substrate 1-Naphtyl phosphate in a micro-chip with low frequency agitation and the product was oxidized at 300 mV. After exposure to formaldehyde, we identified a sharp initial drop in ALP activity followed by an exponential decrease with the exposure time with an effective time constant of ∼30 minutes.",

author = "Carmit Porat-Ophir and Anton Belkin and Sefi Vernick and Vladimir Dergachev and Genrietta Freynd and Mikhail Katsnelson and Yosi Shacham-Diamand",

year = "2013",

doi = "10.1149/2.006312eel",

language = "אנגלית",

volume = "2",

pages = "G8--G10",

journal = "ECS Electrochemistry Letters",

issn = "2162-8726",

publisher = "Electrochemical Society",

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TY - JOUR

T1 - Electrochemical biochip characterization of the effect of formaldehyde on the activity of alkaline phosphatase

AU - Porat-Ophir, Carmit

AU - Belkin, Anton

AU - Vernick, Sefi

AU - Dergachev, Vladimir

AU - Freynd, Genrietta

AU - Katsnelson, Mikhail

AU - Shacham-Diamand, Yosi

PY - 2013

Y1 - 2013

N2 - Alkaline phosphatase (ALP) is a colorectal cancer biomarker that can be electrochemically measured from biopsy samples. Since sampled tissues are usually preserved in formalin, it is crucial to know the effect of formaldehyde on ALP activity in the same biochip used for sampled tissue characterization. ALP was reacted with substrate 1-Naphtyl phosphate in a micro-chip with low frequency agitation and the product was oxidized at 300 mV. After exposure to formaldehyde, we identified a sharp initial drop in ALP activity followed by an exponential decrease with the exposure time with an effective time constant of ∼30 minutes.

AB - Alkaline phosphatase (ALP) is a colorectal cancer biomarker that can be electrochemically measured from biopsy samples. Since sampled tissues are usually preserved in formalin, it is crucial to know the effect of formaldehyde on ALP activity in the same biochip used for sampled tissue characterization. ALP was reacted with substrate 1-Naphtyl phosphate in a micro-chip with low frequency agitation and the product was oxidized at 300 mV. After exposure to formaldehyde, we identified a sharp initial drop in ALP activity followed by an exponential decrease with the exposure time with an effective time constant of ∼30 minutes.

UR - http://www.scopus.com/inward/record.url?scp=84888349448&partnerID=8YFLogxK

U2 - 10.1149/2.006312eel

DO - 10.1149/2.006312eel

M3 - מאמר

AN - SCOPUS:84888349448

VL - 2

SP - G8-G10

JO - ECS Electrochemistry Letters

JF - ECS Electrochemistry Letters

SN - 2162-8726

IS - 12

ER -

Electrochemical biochip characterization of the effect of formaldehyde on the activity of alkaline phosphatase

Abstract

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