Electrochemical biochip characterization of the effect of formaldehyde on the activity of alkaline phosphatase

Carmit Porat-Ophir*, Anton Belkin, Sefi Vernick, Vladimir Dergachev, Genrietta Freynd, Mikhail Katsnelson, Yosi Shacham-Diamand

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Alkaline phosphatase (ALP) is a colorectal cancer biomarker that can be electrochemically measured from biopsy samples. Since sampled tissues are usually preserved in formalin, it is crucial to know the effect of formaldehyde on ALP activity in the same biochip used for sampled tissue characterization. ALP was reacted with substrate 1-Naphtyl phosphate in a micro-chip with low frequency agitation and the product was oxidized at 300 mV. After exposure to formaldehyde, we identified a sharp initial drop in ALP activity followed by an exponential decrease with the exposure time with an effective time constant of ∼30 minutes.

Original languageEnglish
Pages (from-to)G8-G10
JournalECS Electrochemistry Letters
Volume2
Issue number12
DOIs
StatePublished - 2013

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