TY - JOUR
T1 - EGF-like factor epiregulin and amphiregulin expression is regulated by gonadotropins/cAMP in human ovarian follicular cells
AU - Freimann, S.
AU - Ben-Ami, I.
AU - Dantes, A.
AU - Ron-El, R.
AU - Amsterdam, A.
N1 - Funding Information:
This study was supported by grants from the Centers for Excellence in Research and for Women Health at the Weizmann Institute of Science, Rehovot, Israel. A. Amsterdam is the incumbent of the Joyce and Ben B. Eisenberg Professorial Chair in molecular Endocrinology and Cancer Research at the Weizmann Institute of Science.
PY - 2004/11/12
Y1 - 2004/11/12
N2 - Epiregulin and amphiregulin are growth factors involved in cancer development, but their potential role in signaling in the gonads is still obscure. We report here that basal expression of these growth factors is evident in human granulosa cells obtained from women treated for in vitro fertilization, when examined by RT-PCR using RNA isolated from primary cultures of ovarian granulosa cells. Expression of these factors was elevated concomitantly with elevation of progesterone production in these cells upon stimulation with luteinizing hormone (LH), and to a lesser extent with follicle stimulating hormone (FSH), both essential stimulants for ovulation and luteinization. Epiregulin and amphiregulin gene expression was dose- and time-dependent when measured subsequent to LH stimulation. Moreover, forskolin, which activates adenylate cyclase, was as efficient as LH in stimulating expression of these growth factors. It is suggested that upregulation of the epiregulin and amphiregulin expression is part of the signal transduction pathway which leads to ovulation and luteinization in the human ovary.
AB - Epiregulin and amphiregulin are growth factors involved in cancer development, but their potential role in signaling in the gonads is still obscure. We report here that basal expression of these growth factors is evident in human granulosa cells obtained from women treated for in vitro fertilization, when examined by RT-PCR using RNA isolated from primary cultures of ovarian granulosa cells. Expression of these factors was elevated concomitantly with elevation of progesterone production in these cells upon stimulation with luteinizing hormone (LH), and to a lesser extent with follicle stimulating hormone (FSH), both essential stimulants for ovulation and luteinization. Epiregulin and amphiregulin gene expression was dose- and time-dependent when measured subsequent to LH stimulation. Moreover, forskolin, which activates adenylate cyclase, was as efficient as LH in stimulating expression of these growth factors. It is suggested that upregulation of the epiregulin and amphiregulin expression is part of the signal transduction pathway which leads to ovulation and luteinization in the human ovary.
KW - Amphiregulin
KW - Epiregulin
KW - Gonadotropin
KW - Human granulosa cells
UR - http://www.scopus.com/inward/record.url?scp=5144223360&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.09.129
DO - 10.1016/j.bbrc.2004.09.129
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AN - SCOPUS:5144223360
SN - 0006-291X
VL - 324
SP - 829
EP - 834
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -