EGF-like factor epiregulin and amphiregulin expression is regulated by gonadotropins/cAMP in human ovarian follicular cells

S. Freimann, I. Ben-Ami, A. Dantes, R. Ron-El, A. Amsterdam

Research output: Contribution to journalArticlepeer-review

91 Scopus citations

Abstract

Epiregulin and amphiregulin are growth factors involved in cancer development, but their potential role in signaling in the gonads is still obscure. We report here that basal expression of these growth factors is evident in human granulosa cells obtained from women treated for in vitro fertilization, when examined by RT-PCR using RNA isolated from primary cultures of ovarian granulosa cells. Expression of these factors was elevated concomitantly with elevation of progesterone production in these cells upon stimulation with luteinizing hormone (LH), and to a lesser extent with follicle stimulating hormone (FSH), both essential stimulants for ovulation and luteinization. Epiregulin and amphiregulin gene expression was dose- and time-dependent when measured subsequent to LH stimulation. Moreover, forskolin, which activates adenylate cyclase, was as efficient as LH in stimulating expression of these growth factors. It is suggested that upregulation of the epiregulin and amphiregulin expression is part of the signal transduction pathway which leads to ovulation and luteinization in the human ovary.

Original languageEnglish
Pages (from-to)829-834
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume324
Issue number2
DOIs
StatePublished - 12 Nov 2004
Externally publishedYes

Funding

FundersFunder number
Weizmann Institute of Science, Rehovot, Israel
Israeli Centers for Research Excellence

    Keywords

    • Amphiregulin
    • Epiregulin
    • Gonadotropin
    • Human granulosa cells

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