@article{6ec74ba593134e0cae566ca1b775c06c,
title = "Efficient procedure of preparation and properties of long uniform G4-DNA nanowires",
abstract = "Here we describe a novel and efficient procedure for preparation of long uniform G4-DNA wires. The procedure includes (i) enzymatic synthesis of double-stranded DNA molecules consisting of long (up to 10,000 bases), continuous G strands and chains of complementary (dC)20-oligonucleotides, poly(dG)-n(dC)20; (ii) size exclusion HPLC separation of the G strands from the (dC)20 oligonucleotides in 0.1 M NaOH; and (iii) folding of the purified G strands into G4-DNA structures by lowering the pH to 7.0. We show by atomic force microscopy (AFM) that the preparation procedure yielded G4-DNA wires with a uniform morphology and a narrow length distribution. The correlation between the total amount of nucleotides in the G strands and the contour length of the G4-DNA molecules estimated by AFM suggests monomolecular folding of the G strands into quadruplex structures. The folding takes place either in the presence or in the absence of stabilizing ions (K+ or Na+). The addition of these cations leads to a dramatic change in the circular dichroism spectrum of the G4-DNA.",
keywords = "AFM, CD spectroscopy, Enzymatic synthesis, Folding of poly(dG) strands, G4-DNA nanowires",
author = "Natalia Borovok and Tatiana Molotsky and Jamal Ghabboun and Danny Porath and Alexander Kotlyar",
note = "Funding Information: This work was supported by European Grants for Future and Emerging Technologies (IST-2001-38951 [“DNA-Based Nanowires”] and FP6-291292 [“DNA-Based Nanodevices”]).",
year = "2008",
month = mar,
day = "1",
doi = "10.1016/j.ab.2007.10.017",
language = "אנגלית",
volume = "374",
pages = "71--78",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",
}