Efficient FLP recombination in mouse ES cells and oocytes

Julia Schaft; Ruth Ashery-Padan; Frank Der Van Hoeven; Peter Gruss; A. Francis Stewart

doi:10.1002/gene.1076

Efficient FLP recombination in mouse ES cells and oocytes

Julia Schaft, Ruth Ashery-Padan, Frank Der Van Hoeven, Peter Gruss, A. Francis Stewart

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.

Original language	English
Pages (from-to)	6-10
Number of pages	5
Journal	Genesis
Volume	31
Issue number	1
DOIs	https://doi.org/10.1002/gene.1076
State	Published - 2001
Externally published	Yes

Keywords

Cre
Genetic engineering
Homologous recombination
Site specific recombination
Transgenesis

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10.1002/gene.1076

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AU - Ashery-Padan, Ruth

AU - Van Hoeven, Frank Der

AU - Gruss, Peter

AU - Francis Stewart, A.

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AB - We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.

KW - Cre

KW - Genetic engineering

KW - Homologous recombination

KW - Site specific recombination

KW - Transgenesis

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Efficient FLP recombination in mouse ES cells and oocytes

Abstract

Keywords

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