Efficacy of iontophoresis in the rat cornea

Joseph Frucht-Pery*, Arieh Solomon, Ran Doron, Pnina Ever-Hadani, Orly Manor, Mervyn Shapiro

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Iontophoresis can enhance penetration of drugs into tissues. We examined the extent of penetration of gentamicin into the cornea of rats during iontophoresis and the effect of varying the concentrations of gentamicin, the duration of iontophoresis and the current densities during iontophoresis. Methods: Eight groups of rats underwent corneal or iontophoresis using gentamicin dissolved in agar. Low and high concentrations of gentamicin were used, as well as low and high current densities and long and short durations of iontophoresis. Control groups received topical or subconjunctival gentamicin, topical saline solution and mock iontophoresis with the agar-gentamicin mixture. The Mann-Whitney test was used for statistical evaluation. Results: Highly bactericidal concentrations of gentamicin were obtained in all the iontophoresis-treated corneas. The high concentration compared to the low concentration of gentamicin in agar significantly increased the concentration of gentamicin in the corneas, as did the longer duration of iontophoresis. However, higher current intensity did not significantly enhance the drug concentration in the cornea. Conclusion: Iontophoresis with a concentrated gentamicin-agar mixture may provide a rapid increase of gentamicin levels in the cornea.

Original languageEnglish
Pages (from-to)765-769
Number of pages5
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume234
Issue number12
DOIs
StatePublished - Dec 1996
Externally publishedYes

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