@article{0ddfd39a2d5141208bee0ea775bb9eaf,
title = "Effects of recombinant human granulocyte and granulocyte-macrophage colony-stimulating factors on neutrophil function following autologous bone marrow transplantation",
abstract = "Functional activity of peripheral blood neutrophils was assessed in eight patients at 4, 6, 8, 10 and 12 weeks following autologous bone marrow transplantation (ABMT). Functions studied included superoxide generation (O2-) intracellular killing of Staphylococcus aureus, phagocytosis and killing of Candida albicans. Neutrophils were tested following in vitro preincubation with 300 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.2 nM granulocyte colony-stimulating factor (G-CSF) or buffered solution (diluent) as control. Our data indicate that during the early period (weeks 4-6) following ABMT most of the patients exhibited diminished neutrophil oxidative metabolism, defective phagocytosis and killing of C. albicans and reduced capacity to kill S. aureus. In some patients a gradual increase in the functional activity of neutrophils occurred with time. Both GM-CSF and G-CSF induced in vitro amplification of (a) O2- production in response to fmet-leu-phe (FMLP) (b) phagocytosis and killing of C. albicans and (c) killing of S. aureus. This study suggests that GM-CSF and G-CSF may enhance the depressed functional activity of neutrophils following ABMT.",
keywords = "ABMT, G-CSF, GM-CSF, neutrophils",
author = "Miriam Gadish and Yehudith Kletter and Orna Flidel and Arnon Nagler and Shimon Slavin and Ina Fabian",
note = "Funding Information: MYEI.()StiPPRESSION is a common and potentially seriousc omplicationo f the majorityo f anti-cancer agentsb ecauseo f lack of specificityf or malignant cells. One approachto overcomed osel imitationo f chemotherapeuatigce ntsis autologoubso nemarrow * This work was supportedb v Grant 860-0056/3fr om the United States-israeBl inati~.mSalc ienccF oundation (BSF). JerusalemI.s rael.T hiss tudyw asp resenteidn part at the AmericanS ocietyo f \[l ematologmy eetingB. oston. l)eccmbe1r 99((I Blood74, 539a,1 9901. Abbret#ationAs :B MT, autologoubso nem arrowt rans-plantationA; LL, acutel ymphoblastilce ukemiaA: ML, acutem veloidl eukemiaC: . albicans,C andidaa lbicans': CR, completere missionF: CS. fetal calf serum:J MLP. fmet-leu-pheG: -S('F, granulocytec olony-stinmlating factor:G M-CSF, granulocyte-macrophcaogloen y-stimu-latingf actor:H BSS, llank's balancedsa lt solution:H I), I Iodgkin'sd iseaseN: HI,, non-tlo dgkin'sly mphomaP:B S, phosphate-bufferseadli ne: PR, partial remission:R R, respondinrge lapseS: . aureusS, taphylococcuasu reus. Correspondentcoe: Dr lna FabianP, h.D.,H eadD epart-mento f t listologya nd (_'ellB iology,S acklerS choolo f MedicineT. el Aviv UniversityR, amatA viv 69978I,s ract.",
year = "1991",
doi = "10.1016/0145-2126(91)90187-X",
language = "אנגלית",
volume = "15",
pages = "1175--1182",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Ltd.",
number = "12",
}