Effects of recombinant human granulocyte and granulocyte-macrophage colony-stimulating factors on neutrophil function following autologous bone marrow transplantation

Miriam Gadish; Yehudith Kletter; Orna Flidel; Arnon Nagler; Shimon Slavin; Ina Fabian

doi:10.1016/0145-2126(91)90187-X

Effects of recombinant human granulocyte and granulocyte-macrophage colony-stimulating factors on neutrophil function following autologous bone marrow transplantation

Miriam Gadish, Yehudith Kletter, Orna Flidel, Arnon Nagler, Shimon Slavin, Ina Fabian^*

^*Corresponding author for this work

Cell and Developmental Biology

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Functional activity of peripheral blood neutrophils was assessed in eight patients at 4, 6, 8, 10 and 12 weeks following autologous bone marrow transplantation (ABMT). Functions studied included superoxide generation (O₂^-) intracellular killing of Staphylococcus aureus, phagocytosis and killing of Candida albicans. Neutrophils were tested following in vitro preincubation with 300 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.2 nM granulocyte colony-stimulating factor (G-CSF) or buffered solution (diluent) as control. Our data indicate that during the early period (weeks 4-6) following ABMT most of the patients exhibited diminished neutrophil oxidative metabolism, defective phagocytosis and killing of C. albicans and reduced capacity to kill S. aureus. In some patients a gradual increase in the functional activity of neutrophils occurred with time. Both GM-CSF and G-CSF induced in vitro amplification of (a) O₂^- production in response to fmet-leu-phe (FMLP) (b) phagocytosis and killing of C. albicans and (c) killing of S. aureus. This study suggests that GM-CSF and G-CSF may enhance the depressed functional activity of neutrophils following ABMT.

Original language	English
Pages (from-to)	1175-1182
Number of pages	8
Journal	Leukemia Research
Volume	15
Issue number	12
DOIs	https://doi.org/10.1016/0145-2126(91)90187-X
State	Published - 1991

Funding

Funders	Funder number
United States-israeBl

Keywords

ABMT
G-CSF
GM-CSF
neutrophils

Access to Document

10.1016/0145-2126(91)90187-X

Cite this

@article{0ddfd39a2d5141208bee0ea775bb9eaf,

title = "Effects of recombinant human granulocyte and granulocyte-macrophage colony-stimulating factors on neutrophil function following autologous bone marrow transplantation",

abstract = "Functional activity of peripheral blood neutrophils was assessed in eight patients at 4, 6, 8, 10 and 12 weeks following autologous bone marrow transplantation (ABMT). Functions studied included superoxide generation (O2-) intracellular killing of Staphylococcus aureus, phagocytosis and killing of Candida albicans. Neutrophils were tested following in vitro preincubation with 300 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.2 nM granulocyte colony-stimulating factor (G-CSF) or buffered solution (diluent) as control. Our data indicate that during the early period (weeks 4-6) following ABMT most of the patients exhibited diminished neutrophil oxidative metabolism, defective phagocytosis and killing of C. albicans and reduced capacity to kill S. aureus. In some patients a gradual increase in the functional activity of neutrophils occurred with time. Both GM-CSF and G-CSF induced in vitro amplification of (a) O2- production in response to fmet-leu-phe (FMLP) (b) phagocytosis and killing of C. albicans and (c) killing of S. aureus. This study suggests that GM-CSF and G-CSF may enhance the depressed functional activity of neutrophils following ABMT.",

keywords = "ABMT, G-CSF, GM-CSF, neutrophils",

author = "Miriam Gadish and Yehudith Kletter and Orna Flidel and Arnon Nagler and Shimon Slavin and Ina Fabian",

note = "Funding Information: MYEI.()StiPPRESSION is a common and potentially seriousc omplicationo f the majorityo f anti-cancer agentsb ecauseo f lack of specificityf or malignant cells. One approachto overcomed osel imitationo f chemotherapeuatigce ntsis autologoubso nemarrow * This work was supportedb v Grant 860-0056/3fr om the United States-israeBl inati~.mSalc ienccF oundation (BSF). JerusalemI.s rael.T hiss tudyw asp resenteidn part at the AmericanS ocietyo f \[l ematologmy eetingB. oston. l)eccmbe1r 99((I Blood74, 539a,1 9901. Abbret#ationAs :B MT, autologoubso nem arrowt rans-plantationA; LL, acutel ymphoblastilce ukemiaA: ML, acutem veloidl eukemiaC: . albicans,C andidaa lbicans': CR, completere missionF: CS. fetal calf serum:J MLP. fmet-leu-pheG: -S('F, granulocytec olony-stinmlating factor:G M-CSF, granulocyte-macrophcaogloen y-stimu-latingf actor:H BSS, llank's balancedsa lt solution:H I), I Iodgkin'sd iseaseN: HI,, non-tlo dgkin'sly mphomaP:B S, phosphate-bufferseadli ne: PR, partial remission:R R, respondinrge lapseS: . aureusS, taphylococcuasu reus. Correspondentcoe: Dr lna FabianP, h.D.,H eadD epart-mento f t listologya nd (_'ellB iology,S acklerS choolo f MedicineT. el Aviv UniversityR, amatA viv 69978I,s ract.",

year = "1991",

doi = "10.1016/0145-2126(91)90187-X",

language = "אנגלית",

volume = "15",

pages = "1175--1182",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier Ltd.",

number = "12",

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TY - JOUR

T1 - Effects of recombinant human granulocyte and granulocyte-macrophage colony-stimulating factors on neutrophil function following autologous bone marrow transplantation

AU - Gadish, Miriam

AU - Kletter, Yehudith

AU - Flidel, Orna

AU - Nagler, Arnon

AU - Slavin, Shimon

AU - Fabian, Ina

N1 - Funding Information: MYEI.()StiPPRESSION is a common and potentially seriousc omplicationo f the majorityo f anti-cancer agentsb ecauseo f lack of specificityf or malignant cells. One approachto overcomed osel imitationo f chemotherapeuatigce ntsis autologoubso nemarrow * This work was supportedb v Grant 860-0056/3fr om the United States-israeBl inati~.mSalc ienccF oundation (BSF). JerusalemI.s rael.T hiss tudyw asp resenteidn part at the AmericanS ocietyo f \[l ematologmy eetingB. oston. l)eccmbe1r 99((I Blood74, 539a,1 9901. Abbret#ationAs :B MT, autologoubso nem arrowt rans-plantationA; LL, acutel ymphoblastilce ukemiaA: ML, acutem veloidl eukemiaC: . albicans,C andidaa lbicans': CR, completere missionF: CS. fetal calf serum:J MLP. fmet-leu-pheG: -S('F, granulocytec olony-stinmlating factor:G M-CSF, granulocyte-macrophcaogloen y-stimu-latingf actor:H BSS, llank's balancedsa lt solution:H I), I Iodgkin'sd iseaseN: HI,, non-tlo dgkin'sly mphomaP:B S, phosphate-bufferseadli ne: PR, partial remission:R R, respondinrge lapseS: . aureusS, taphylococcuasu reus. Correspondentcoe: Dr lna FabianP, h.D.,H eadD epart-mento f t listologya nd (_'ellB iology,S acklerS choolo f MedicineT. el Aviv UniversityR, amatA viv 69978I,s ract.

PY - 1991

Y1 - 1991

N2 - Functional activity of peripheral blood neutrophils was assessed in eight patients at 4, 6, 8, 10 and 12 weeks following autologous bone marrow transplantation (ABMT). Functions studied included superoxide generation (O2-) intracellular killing of Staphylococcus aureus, phagocytosis and killing of Candida albicans. Neutrophils were tested following in vitro preincubation with 300 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.2 nM granulocyte colony-stimulating factor (G-CSF) or buffered solution (diluent) as control. Our data indicate that during the early period (weeks 4-6) following ABMT most of the patients exhibited diminished neutrophil oxidative metabolism, defective phagocytosis and killing of C. albicans and reduced capacity to kill S. aureus. In some patients a gradual increase in the functional activity of neutrophils occurred with time. Both GM-CSF and G-CSF induced in vitro amplification of (a) O2- production in response to fmet-leu-phe (FMLP) (b) phagocytosis and killing of C. albicans and (c) killing of S. aureus. This study suggests that GM-CSF and G-CSF may enhance the depressed functional activity of neutrophils following ABMT.

AB - Functional activity of peripheral blood neutrophils was assessed in eight patients at 4, 6, 8, 10 and 12 weeks following autologous bone marrow transplantation (ABMT). Functions studied included superoxide generation (O2-) intracellular killing of Staphylococcus aureus, phagocytosis and killing of Candida albicans. Neutrophils were tested following in vitro preincubation with 300 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.2 nM granulocyte colony-stimulating factor (G-CSF) or buffered solution (diluent) as control. Our data indicate that during the early period (weeks 4-6) following ABMT most of the patients exhibited diminished neutrophil oxidative metabolism, defective phagocytosis and killing of C. albicans and reduced capacity to kill S. aureus. In some patients a gradual increase in the functional activity of neutrophils occurred with time. Both GM-CSF and G-CSF induced in vitro amplification of (a) O2- production in response to fmet-leu-phe (FMLP) (b) phagocytosis and killing of C. albicans and (c) killing of S. aureus. This study suggests that GM-CSF and G-CSF may enhance the depressed functional activity of neutrophils following ABMT.

KW - ABMT

KW - G-CSF

KW - GM-CSF

KW - neutrophils

UR - http://www.scopus.com/inward/record.url?scp=0026326396&partnerID=8YFLogxK

U2 - 10.1016/0145-2126(91)90187-X

DO - 10.1016/0145-2126(91)90187-X

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AN - SCOPUS:0026326396

SN - 0145-2126

VL - 15

SP - 1175

EP - 1182

JO - Leukemia Research

JF - Leukemia Research

IS - 12

ER -

Effects of recombinant human granulocyte and granulocyte-macrophage colony-stimulating factors on neutrophil function following autologous bone marrow transplantation

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