Effects of Pi and ADP on ATPase activity in chloroplasts

C. Carmeli*, Y. Lifshitz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


1. 1. The rate of decay of the light-triggered state of ATPase in chloroplasts was decreased in the presence of Pi and accelerated by ADP in a phosphateless medium. Phosphate and arsenate inhibited the effect of ADP. 2. 2. Compared with other dinucleotides, ADP was found to be highly specific for the acceleration of the decay, having an apparent Km of 1.1·10-6 M. A lower apparent Km for ADP was obtained in the presence of phosphate. The apparent Km for the inhibition of the effect of ADP by Pi was lowered with increased concentrations of ADP. 3. 3. These and other effects on ATPase and ATP-Pi exchange activities were discussed in terms of changes in the permeability of ADP and Pi across the membrane of the chloroplast.

Original languageEnglish
Pages (from-to)86-95
Number of pages10
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number1
StatePublished - 20 Apr 1972


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