Effects of chlorocyclizine on pulmonary lipid metabolism in rats

Nora Stern, Aliza Tietz, Edith Gaton, Moshe Wolman

Research output: Contribution to journalArticlepeer-review

Abstract

Pulmonary lipidosis was induced in rats by including 0.36 and 0.54% chlorocyclizine in their diet. Chemical analyses of the lung tissue revealed a very marked increase in phosphatidylcholine concentration. Phosphatidylglycerol and phosphatidylinositol concentrations were also markedly increased. An increase in the phosphatidylcholine content was also observed in lavage fluid and macrophages. Microscopic examination of the cell fraction showed that almost all the cells of the lavage fluid were macrophages and that histochemically demonstrable acid esterase activity was mostly inversely related to storage of lipids in the cells. Sonication of macrophages isolated from normal or chlorocyclizine-treated rats yielded a soluble acid phospholipase (pH optimum, 4.0) and a neutral (pH optimum, 8.2) membrane-bound, CaCl2-dependent enzyme. An inhibitory effect of chlorocyclizine in vitro on the activity of the soluble phospholipase was shown.

Original languageEnglish
Pages (from-to)166-173
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume754
Issue number2
DOIs
StatePublished - 29 Nov 1983

Keywords

  • (Rat lung)
  • Acid phospholipase
  • Chlorocyclizine
  • Pulmonary lipidosis

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