Effect of the pregnane-related GABA-active steroid alphaxalone on mice performance in the staircase test

Chaim G. Pick*, Yakov Peter, Lior Paz, Shaul Schreiber, Moshe Gavish, Ronit Weizman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We evaluated the modulatory effect of the GABA-active nenrosteroid alphaxalone on the staircase test behavior of mice. Results were compared with the benzodiazepine alprazolam, the GABA(A) agonist muscimol and the peripheral steroids corticosterone and progesterone. Alphaxalone and alprazolam reduced rearing activity in a dose-dependent manner, at doses that did not suppress climbing. The rearing-suppression effect of alprazolam, but not of alphaxalone, was blocked by the benzodiazepine antagonist flumazenil. No such dissociation between the effect on rearing and climbing was obtained with muscimol, and both activities were suppressed, in a flumazenil- insensitive pattern, at high doses. Corticosterone and progesterone did not affect the behavior of the mice. The lack of sensitivity of both phenobarbital and alphaxalone to flumazenil indicates that neither agents act via the benzodiazepine recognition site at the GABA(A) receptor complex.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalBrain Research
Volume765
Issue number1
DOIs
StatePublished - 8 Aug 1997

Keywords

  • Alphaxalone
  • Alprazolam
  • Anxiolytic
  • Flumazenil
  • GABA(A) receptor
  • Muscimol
  • Neuroactive steroid
  • Staircase test

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