Abstract
Administration of clomiphene citrate to rats may cause an arrest of the spermatic process by interfering with pituitary-gonadalaxis. Well recognized is the impaired sperm production after chemotherapy treatment. The enzyme sialyltransferase participates in a variety of sialoglycolipid's and sialogyprotein's biosynthetic processes including the process of fertilization. This study was conducted to evaluate the effect of clomiphene citrate, procarbazine and clomiphene citrate plus procarbazine administration on the activity of sialyltransferase in homogenates of adult rats testis/epididymis. The enzyme activity, based on measurement of radioactivity incorporated into asialofetuin after incubation of tissue homogenates in the presence of CMP-(C14 sialic acid) was expressed as cpm per 167 mg tissue. Following treatment the activity of sialyltransferase in testis homogenates was elevated while in the epididymis the activity decreased. After 8 weeks recovery period the enzyme activity in both tissues was almost normal. It would appear that the deleterious effect of clomiphene citrate and procarbazine on sialyltransferase activity in both tissues is of transient nature and not correlated with the status of spermatogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 107-111 |
| Number of pages | 5 |
| Journal | Advances in Contraceptive Delivery Systems |
| Volume | 9 |
| Issue number | 2-3 |
| State | Published - 1993 |