Effect of pH on mutagenesis by thiols in Salmonella typhimurium TA102

Avishay Abraham Stark; Aya Arad; Sandra Siskindovich; Dennis A. Pagano; Errol Zeiger

doi:10.1016/0165-1218(89)90007-4

Effect of pH on mutagenesis by thiols in Salmonella typhimurium TA102

Avishay Abraham Stark^*, Aya Arad, Sandra Siskindovich, Dennis A. Pagano, Errol Zeiger

^*Corresponding author for this work

Biochemistry Molecular Biology

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Abstract

The mutagenicity of thiol (SH)-containing compounds was tested in Salmonella typhimurium TA102 in the liquid preincubation method. Cysteinyl-glycine (CG), cysteine ethyl ester (CEE), l- and d-penicillamine (PA), cysteine (Cys) and glutathione (GSH) were mutagenic to strain TA102 without metabolic activation. On a molar basis, CG was the most potent mutagen. The mutagenicity of the remaining compounds decreased in the order specified above. The mutagenic response of each thiol-containing compound was a function of the pK_a of the thiol group and the pH of the preincubation mixture. This indicates that a thiolate anion, rather than a free thiol, is required for mutagenesis.

Original language	English
Pages (from-to)	89-94
Number of pages	6
Journal	Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
Volume	224
Issue number	1
DOIs	https://doi.org/10.1016/0165-1218(89)90007-4
State	Published - Sep 1989

Keywords

Salmonella typhimurium
Thiols
pH, effect

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10.1016/0165-1218(89)90007-4

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AU - Zeiger, Errol

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Effect of pH on mutagenesis by thiols in Salmonella typhimurium TA102

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