Effect of pH on mutagenesis by thiols in Salmonella typhimurium TA102

Avishay Abraham Stark*, Aya Arad, Sandra Siskindovich, Dennis A. Pagano, Errol Zeiger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The mutagenicity of thiol (SH)-containing compounds was tested in Salmonella typhimurium TA102 in the liquid preincubation method. Cysteinyl-glycine (CG), cysteine ethyl ester (CEE), l- and d-penicillamine (PA), cysteine (Cys) and glutathione (GSH) were mutagenic to strain TA102 without metabolic activation. On a molar basis, CG was the most potent mutagen. The mutagenicity of the remaining compounds decreased in the order specified above. The mutagenic response of each thiol-containing compound was a function of the pKa of the thiol group and the pH of the preincubation mixture. This indicates that a thiolate anion, rather than a free thiol, is required for mutagenesis.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalMutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
Volume224
Issue number1
DOIs
StatePublished - Sep 1989

Keywords

  • Salmonella typhimurium
  • Thiols
  • pH, effect

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