Effect of ouabain on electrical conductance of frog skin. Evidence against recycling of sodium

Ayus Corcia, Judith Lahav, S. Roy Caplan

Research output: Contribution to journalArticlepeer-review

Abstract

Ouabain (10-4 M) added to the serosal solution of isolated frog skin not only stops the active transport of Na+ but causes a dramatic increase in the electric conductance of the skin. The effect also appears when ouabain is added to amiloride-pretreated skins, ruling out the possibility of a cellular effect. The similarity between the effect of ouabain on normal and amiloride-pretreated skins indicates that no appreciable recycling of Na+ across the basolateral membrane of frog skin takes place. The change of Na+ efflux after ouabain is added to amiloride-pretreated skins parallels the change of electric conductance, indicating that besides blocking the Na+ pump, ouabain affects the paracellular shunt pathway between the cells.

Original languageEnglish
Pages (from-to)264-271
Number of pages8
JournalBBA - Biomembranes
Volume596
Issue number2
DOIs
StatePublished - 28 Feb 1980
Externally publishedYes

Keywords

  • (Frog skin)
  • Amiloride
  • Electrical conductance
  • Na efflux
  • Ouabain

Fingerprint

Dive into the research topics of 'Effect of ouabain on electrical conductance of frog skin. Evidence against recycling of sodium'. Together they form a unique fingerprint.

Cite this