Effect of ouabain on electrical conductance of frog skin. Evidence against recycling of sodium

Ayus Corcia; Judith Lahav; S. Roy Caplan

doi:10.1016/0005-2736(80)90360-0

Effect of ouabain on electrical conductance of frog skin. Evidence against recycling of sodium

Ayus Corcia^*, Judith Lahav, S. Roy Caplan

^*Corresponding author for this work

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Ouabain (10^-4 M) added to the serosal solution of isolated frog skin not only stops the active transport of Na⁺ but causes a dramatic increase in the electric conductance of the skin. The effect also appears when ouabain is added to amiloride-pretreated skins, ruling out the possibility of a cellular effect. The similarity between the effect of ouabain on normal and amiloride-pretreated skins indicates that no appreciable recycling of Na⁺ across the basolateral membrane of frog skin takes place. The change of Na⁺ efflux after ouabain is added to amiloride-pretreated skins parallels the change of electric conductance, indicating that besides blocking the Na⁺ pump, ouabain affects the paracellular shunt pathway between the cells.

Original language	English
Pages (from-to)	264-271
Number of pages	8
Journal	BBA - Biomembranes
Volume	596
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(80)90360-0
State	Published - 28 Feb 1980
Externally published	Yes

Keywords

(Frog skin)
Amiloride
Electrical conductance
Na efflux
Ouabain

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10.1016/0005-2736(80)90360-0

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title = "Effect of ouabain on electrical conductance of frog skin. Evidence against recycling of sodium",

abstract = "Ouabain (10-4 M) added to the serosal solution of isolated frog skin not only stops the active transport of Na+ but causes a dramatic increase in the electric conductance of the skin. The effect also appears when ouabain is added to amiloride-pretreated skins, ruling out the possibility of a cellular effect. The similarity between the effect of ouabain on normal and amiloride-pretreated skins indicates that no appreciable recycling of Na+ across the basolateral membrane of frog skin takes place. The change of Na+ efflux after ouabain is added to amiloride-pretreated skins parallels the change of electric conductance, indicating that besides blocking the Na+ pump, ouabain affects the paracellular shunt pathway between the cells.",

keywords = "(Frog skin), Amiloride, Electrical conductance, Na efflux, Ouabain",

author = "Ayus Corcia and Judith Lahav and Caplan, {S. Roy}",

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AU - Corcia, Ayus

AU - Lahav, Judith

AU - Caplan, S. Roy

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PY - 1980/2/28

Y1 - 1980/2/28

N2 - Ouabain (10-4 M) added to the serosal solution of isolated frog skin not only stops the active transport of Na+ but causes a dramatic increase in the electric conductance of the skin. The effect also appears when ouabain is added to amiloride-pretreated skins, ruling out the possibility of a cellular effect. The similarity between the effect of ouabain on normal and amiloride-pretreated skins indicates that no appreciable recycling of Na+ across the basolateral membrane of frog skin takes place. The change of Na+ efflux after ouabain is added to amiloride-pretreated skins parallels the change of electric conductance, indicating that besides blocking the Na+ pump, ouabain affects the paracellular shunt pathway between the cells.

AB - Ouabain (10-4 M) added to the serosal solution of isolated frog skin not only stops the active transport of Na+ but causes a dramatic increase in the electric conductance of the skin. The effect also appears when ouabain is added to amiloride-pretreated skins, ruling out the possibility of a cellular effect. The similarity between the effect of ouabain on normal and amiloride-pretreated skins indicates that no appreciable recycling of Na+ across the basolateral membrane of frog skin takes place. The change of Na+ efflux after ouabain is added to amiloride-pretreated skins parallels the change of electric conductance, indicating that besides blocking the Na+ pump, ouabain affects the paracellular shunt pathway between the cells.

KW - (Frog skin)

KW - Amiloride

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KW - Na efflux

KW - Ouabain

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Effect of ouabain on electrical conductance of frog skin. Evidence against recycling of sodium

Abstract

Keywords

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