TY - JOUR
T1 - Effect of oral colchicine on T cell subsets, monocytes and concanavalin A‐induced suppressor cell function in asthmatic patients
AU - ILFELD, D. N.
AU - MAZAR, A.
AU - GARTY, M.
AU - FINK, G.
AU - SPITZER, S.
AU - PECHT, M.
AU - NETZER, L.
AU - TRAININ, N.
AU - KUPERMAN, O.
PY - 1986/9
Y1 - 1986/9
N2 - Asthmatic patients have a deficiency of concanavalin A‐(Con A) induced suppressor cell function. We tested whether oral colchicine 0·5 mg twice daily for 7 days could correct this immunoregulatory abnormality. Peripheral blood mononuclear cells were incubated with Con A and then suppression of proliferation was measured by co‐culture of these cells with healthy volunteers’mononuclear cells and phytohaemagglutinin. Sixteen asthmatic patients had significantly (P < 0·002) decreased Con A‐induced suppressor cell function (17·0±17·2%, mean ± s.d.) as compared to 13 healthy volunteers (37·9±14·9%). Oral colchicine significantly (P < 0·05) increased, though only partially corrected, these 16 asthmatic patients’Con A‐induced suppressor cell function (28·1±14·3%). Asthmatic patients had an increased number of monocytes (691±289 vs 388±271/mm3 for normals, P < 0·01) and a normal number of lymphocytes, Leu 4+ total T cells, Leu 3+ helper/inducer T cells, and Leu 2+ suppressor/cytotoxic T cells as well as a normal Leu 3/Leu 2 ratio. Oral colchicine significantly (P < 0·005) decreased the number of monocytes (451±255/mm3) without significantly affecting the number of lymphocytes, Leu 4+, Leu 3+, or Leu 2+ T cells, or the Leu 3/Leu 2 ratio. These results are consistent with the hypothesis that the deficiency of Con A‐induced suppressor cell function in asthmatic patients may be due, in part, to an increased number and/or abnormal activity of monocytes. If so, then oral colchicine may have partially corrected the deficiency of Con A‐induced suppressor cell function by decreasing the number and/or modulating the activity of monocytes.
AB - Asthmatic patients have a deficiency of concanavalin A‐(Con A) induced suppressor cell function. We tested whether oral colchicine 0·5 mg twice daily for 7 days could correct this immunoregulatory abnormality. Peripheral blood mononuclear cells were incubated with Con A and then suppression of proliferation was measured by co‐culture of these cells with healthy volunteers’mononuclear cells and phytohaemagglutinin. Sixteen asthmatic patients had significantly (P < 0·002) decreased Con A‐induced suppressor cell function (17·0±17·2%, mean ± s.d.) as compared to 13 healthy volunteers (37·9±14·9%). Oral colchicine significantly (P < 0·05) increased, though only partially corrected, these 16 asthmatic patients’Con A‐induced suppressor cell function (28·1±14·3%). Asthmatic patients had an increased number of monocytes (691±289 vs 388±271/mm3 for normals, P < 0·01) and a normal number of lymphocytes, Leu 4+ total T cells, Leu 3+ helper/inducer T cells, and Leu 2+ suppressor/cytotoxic T cells as well as a normal Leu 3/Leu 2 ratio. Oral colchicine significantly (P < 0·005) decreased the number of monocytes (451±255/mm3) without significantly affecting the number of lymphocytes, Leu 4+, Leu 3+, or Leu 2+ T cells, or the Leu 3/Leu 2 ratio. These results are consistent with the hypothesis that the deficiency of Con A‐induced suppressor cell function in asthmatic patients may be due, in part, to an increased number and/or abnormal activity of monocytes. If so, then oral colchicine may have partially corrected the deficiency of Con A‐induced suppressor cell function by decreasing the number and/or modulating the activity of monocytes.
UR - http://www.scopus.com/inward/record.url?scp=85004857896&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2222.1986.tb01975.x
DO - 10.1111/j.1365-2222.1986.tb01975.x
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AN - SCOPUS:85004857896
SN - 0954-7894
VL - 16
SP - 407
EP - 416
JO - Clinical and Experimental Allergy
JF - Clinical and Experimental Allergy
IS - 5
ER -
