Effect of magnesium sulfate concentration on serum ionized magnesium in vitro

Mia Salamon, Francis B. Mimouni, Shaul Dollberg, Dror Mandel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


We undertook this in vitro study to quantify the effect that addition of magnesium (Mg) sulfate to neonatal serum, as compared with adult serum, might have on ionized Mg (Mg2+) concentration. We used one cord blood sample and one adult blood sample that we made hypermagnesemic by adding various amounts of magnesium sulfate to study five levels of serum Mg. Each sample was then studied at one of three levels of pH, from extreme alkalosis to extreme acidosis. We measured the changes in serum Mg2+ and serum ionized calcium (Ca2+) in reaction to these changes in pH and Mg. At each pH level, there was an exponential increase in the serum Mg2+ (and no significant change in serum Ca2+) with increasing serum Mg. Multiple regression analysis using Mg2+ as the dependent variable and baseline Ca2+, phosphorous, albumin, and blood type (adult versus cord blood) as well as blood pH and serum Mg as independent variables indicated that serum Mg and pH were the only variables significantly influencing serum Mg 2+. Within the range of serum Mg considered, the addition of magnesium sulfate in vitro causes an exponential increase in Mg2+ and no significant change in serum Ca2+.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalAmerican Journal of Perinatology
Issue number7
StatePublished - 2010


  • Magnesium supplementation
  • ionized magnesium


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