Effect of intralipid on the phagocytic and microbicidal capacity of human monocytes in culture

S. Padeh, R. Shor, J. H. Passwell

Research output: Contribution to journalArticlepeer-review

Abstract

We studied the effect of Intralipid (IL) in monocyte cultures based on the ability of the cultures to phagocytose and kill Candida albicans and produce the oxidative burst. The IL was taken up by monocytes in cultures, and these cells phagocytosed more Candida organisms than did the control cells [85 ± 2.2% in the IL treated (1%) compared to 68 ± 23% after 1 h in the control). The percentage of killing of Candida albicans, which had been taken up by the IL-treated monocytes measured after 2 h in culture (48.3 ± 6.0%), was no different when compared to control (47.0 ± 5.8%). Following ingestion of IL. there was an increase in basal H202 production, however, the presence of the IL in the cells had no effect on the expected increase in H202 production following stimulation with either phorbol myristate acetate (PMA) or zymosan particles. Compared to untreated cells, a significant increase in the number of monocytes with positive nitroblue tetrazolium staining was observed in monocytes that had ingested IL (when they were stimulated with either PMA or Candida microorganisms). Similar results were obtained in monocyte-derived macrophages (i.e., monocytes in monolayer cultures for 10 days). These findings suggest that the essential monocyte functions of phagocytosis, microbicidal activity, and ability to elicit an oxidative burst are not directly altered by the conventional use of IL in clinical practice.

Original languageEnglish
Pages (from-to)575-580
Number of pages6
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume6
Issue number4
DOIs
StatePublished - 1987

Keywords

  • Candida albicans
  • Intralipid
  • Monocyte
  • Phagocyte

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