Effect of intradermal human recombinant copper-zinc superoxide dismutase on random pattern flaps in rats

Ophir Schein, Melvyn Westreich, Avshalom Shalom

Research output: Contribution to journalArticlepeer-review

Abstract

Background Studies have focused on enhancing flap viability using superoxide dismutase (SOD), but only a few used SOD from human origin, and most gave the compound systemically. We evaluated the ability of SOD to improve random skin flap survival using human recombinant copper-zinc superoxide dismutase (Hr-CuZnSOD) in variable doses, injected intradermally into the flap. Methods Seventy male Sprague Dawley rats were randomly assigned into 4 groups. Cephalic random pattern flaps were elevated on their backs and intradermal injections of different dosages of Hr-CuZnSOD were given 15 minutes before surgery. Flap survival was evaluated by fluorescein fluorescence. Analysis of variance (ANOVA) and t test statistical analyses were performed. Results Flap survival in all treated groups was significantly better than in the controls. Conclusions The beneficial effect of HR-CuZnSOD on flap survival is attained when it is given intradermally into the flap tissue. Theoretically, Hr-CuZnSOD delivered with local anesthetics used in flap elevation may be a valuable clinical tool.

Original languageEnglish
Pages (from-to)1265-1268
Number of pages4
JournalHead and Neck
Volume35
Issue number9
DOIs
StatePublished - Sep 2013

Keywords

  • SOD
  • flaps
  • oxygen free radicals
  • rats
  • survival

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