Effect of insulin and glucagon on the mobility of ESR-probes incorporated in rat liver plasma membranes

I. Zlatanov*, E. Maltzeva, N. Borovok, V. Spassov

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1. The effect of different concentrations of insulin (INS) and glucagon (GLU) on the rotational mobility of a membrane-incorporated spin probe 2,2-6,6-tetramethyl-4-capriloyl piperidine-1-oxil (C7) was investigated by electron spin resonance (ESR) technique. 2. 2. Two strong adenylate cyclase effectors guanosine 5'-triphosphate (GTP) and guanylyl 5'-imidodiphosphate [Gpp(NH)p], as well as an antioxidant 4-methyl-2,6-ditretbutilphenol (AO) and a nonprotein hormone prostaglandin E2 (PGE2) were used as reference effectors. 3. 3. Applied effectors reduced by 30-82% the rotation correlation time (TR) of the rat liver plasma membranes spin probe C7. The effect was time-dependent and reached saturation 30-40 min after the effector application. 4. 4. The kinetic- and concentration-dependent changes in TR were described by a simple phenomenoloical model. The apparent binding constants and the number of the apparent membrane binding sites of the effectors used were calculated using the model.

Original languageEnglish
Pages (from-to)971-977
Number of pages7
JournalInternational Journal of Biochemistry
Volume25
Issue number7
DOIs
StatePublished - Jul 1993

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