Effect of indomethacin on IL-1β, IL-6 and TNFα production by mononuclear cells of preterm newborns and adults

L. Sirota, I. Punsky, H. Bessler

Research output: Contribution to journalArticlepeer-review

Abstract

The in vitro effect of indomethacin (IM) on the production of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)α by cord blood mononuclear cells (CBMC) from preterm newborns was compared to that of peripheral blood MC of adults (PBMC). MC isolated from peripheral blood of adults (PBMC) and cord blood of preterm newborns (CBMC) were incubated without or with lipopolysaccharide (LPS) in the absence or presence of various concentrations of IM. The levels of IL-1β, IL-6 and TNFα in the supernatants were tested by ELISA. MC isolated from preterm newborns were less sensitive to the in vitro effect of IM on IL-1β and TNFα secretion than adult cells. While the spontaneous secretion of IL-1β and TNFα and the production of TNFα induced by LPS were significantly increased following incubation of adult PBMC with IM, only the spontaneous synthesis of TNFα by CBMC of preterm newborns was affected by this drug. The in vitro production of IL-6 by MC in the two groups was not affected by the addition of IM. It is suggested that IM may affect the preterm's immune response. However, the role of the drug in the frequency and severity of infections in the neonatal intensive care Unit needs further investigation.

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalActa Paediatrica, International Journal of Paediatrics
Volume89
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Cytokines
  • Indomethacin
  • Mononuclear cells
  • Preterm newborns

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