TY - JOUR
T1 - Effect of hyperglycemia on sorbitol and myo-inositol content of cultured rat conceptus
T2 - Failure of aldose reductase inhibitors to modify myo-inositol depletion and dysmorphogenesis
AU - Hod, Moshe
AU - Star, Sara
AU - Passonneau, Janet V.
AU - Unterman, Terry G.
AU - Freinkel, Norbert
N1 - Funding Information:
These studies were supported by NIH grants AM10699, MRP HD11021, and Training Grant AM07169 from the National Institutes of Health. Dr. Hod (Beilinson Medical Center, Tel Aviv, Israel) was an Overseas Research Fellow in the Center for Endocrinology, Metabolism and Nutrition of Northwestern University Medical School during 1985-1986.
PY - 1986/11/14
Y1 - 1986/11/14
N2 - Growth retardation and dysmorphogenesis were seen in the rat conceptus following culture from day 9.5 to 11.5 of development in the presence of increased amounts of glucose. Sorbitol accumulated in the conceptus during the hyperglycemia whereas total protein, DNA, and free myo-inositol decreased. Similar glucose-related compositional changes were present in separated embryos and extra-embryonic membranes suggesting that all portions of the conceptus participate in the alterations. The aldose reductase inhibitors Sorbinil and Statil obtunded the rises in sorbitol but did not modify the increased incidence of malformations and the fall in DNA, protein and myo-inositol. Thus, the aldose reductase pathway may function in the early post-implantation rat conceptus but the resultant accumulation of sorbitol does not appear to contribute to the growth-retarding, dysmorphogenic, and myo-inositol-depleting effects of hyperglycemia.
AB - Growth retardation and dysmorphogenesis were seen in the rat conceptus following culture from day 9.5 to 11.5 of development in the presence of increased amounts of glucose. Sorbitol accumulated in the conceptus during the hyperglycemia whereas total protein, DNA, and free myo-inositol decreased. Similar glucose-related compositional changes were present in separated embryos and extra-embryonic membranes suggesting that all portions of the conceptus participate in the alterations. The aldose reductase inhibitors Sorbinil and Statil obtunded the rises in sorbitol but did not modify the increased incidence of malformations and the fall in DNA, protein and myo-inositol. Thus, the aldose reductase pathway may function in the early post-implantation rat conceptus but the resultant accumulation of sorbitol does not appear to contribute to the growth-retarding, dysmorphogenic, and myo-inositol-depleting effects of hyperglycemia.
UR - http://www.scopus.com/inward/record.url?scp=0022978398&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(86)90731-X
DO - 10.1016/0006-291X(86)90731-X
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AN - SCOPUS:0022978398
SN - 0006-291X
VL - 140
SP - 974
EP - 980
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -