Effect of fluorine substitution on sintering behaviour, mechanical and bioactivity of hydroxyapatite

Purnendu Nasker, Aniruddha Samanta, Sudip Rudra, Arijit Sinha, Anoop K. Mukhopadhyay, Mitun Das*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Fluorine substituted hydroxyapatite (FAp) with different degree of fluorine (F) substitution, has been synthesized using hydrothermal synthesis method. In the present work, as synthesized powders were consolidated by sintering at 1200 °C in air for 1 h. The sintered specimens were characterized using Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) for phase analysis. Further, fluorine intake in the sintered specimens was evaluated using ion chromatography (IC). XRD peaks clearly showed biphasic nature of the sintered specimen. However, the sintered samples containing more than ∼60% fluorine substitution showed no β-tricalcium phosphate (β-TCP) phase formation. The IC results revealed that the degree of fluoridation decreased significantly in the sintered specimen compare to the respective as synthesized powders. The effect of actual fluorine content in the sintered specimens was further evaluated in terms of sinterability, surface energy, mechanical properties and in vitro cytocompatibility study. The surface energy of the sintered specimen decreased from 51.8 mN/m to 42.5 mN/m, in which degree of fluoridation varies from 0% to 110%. The in vitro cytocompatibility of the sintered specimen were carried out against mouse osteoblast cell line (MC3T3-E1). In vitro study showed that all the samples were nontoxic but cell proliferation for the samples containing more than 40% fluorine substitution became significantly low.

Original languageEnglish
Pages (from-to)136-142
Number of pages7
JournalJournal of the Mechanical Behavior of Biomedical Materials
StatePublished - Jul 2019
Externally publishedYes


  • Biocompatibility
  • Fluorapatite
  • Hydroxyapatite
  • Phase analysis
  • Surface energy


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