Effect of exogenous recombinant human granulocyte and granulocyte-macrophage colony-stimulating factor on neutrophil function following allogeneic bone marrow transplantation

I. Fabian, Y. Kletter, I. Bleiberg, M. Gadish, E. Naparsteck, S. Slavin

Research output: Contribution to journalArticlepeer-review

Abstract

Functional activity of peripheral blood granulocytes was assessed in seven patients and in their normal donors following allogeneic bone marrow transplantation (BMT). Functions studied included superoxide generation (O2-), intracellular killing of Staphylococcus aureus, phagocytosis, and killing of Candida albicans. Neutrophils were tested following preincubation with 300 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.2 nM granulocyte colony-stimulating factor (G-SCF), or buffered solution (diluent) as control. Our data indicate that following BMT, both recipients and their normal donors show GM-CSF- and G-CSF-induced increases in: 1) O2- production in response to fMet-Leu-Phe (fMLP), 2) killing of S. aureus, and 3) phagocytosis of C. albicans. In two patients that showed low candidacidal activity, GM-CSF and G-CSF markedly enhanced the cytotoxic activity of the cells. Our studies indicate that GM-CSF and G-CSF increase 'oxygen-dependent' oxidative activities in neutrophils from BMT recipients and their normal donors and enhance the antimicrobial activity of the cells.

Original languageEnglish
Pages (from-to)868-873
Number of pages6
JournalExperimental Hematology
Volume19
Issue number9
StatePublished - 1991

Keywords

  • Bone marrow transplantation
  • G-CSF
  • GM-CSF

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