Effect of dexamethasone on IL-10 and IL-12p40 production in newborns and adults

Hanna Bessler; S. Kagazanov; I. Punsky; L. Sirota

doi:10.1159/000047154

Effect of dexamethasone on IL-10 and IL-12p40 production in newborns and adults

Hanna Bessler^*, S. Kagazanov, I. Punsky, L. Sirota

^*Corresponding author for this work

Clinical Departments

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The effect of dexamethasone (DEX) on interleukin (IL)-10 and IL-12p40 production was examined in preterm newborns, term infants and was compared to that in adults. Mononuclear cells isolated from newborn cord blood (CBMC) and peripheral blood from adults (PBMC) were incubated with lipopolysaccharide in the absence or presence of DEX at concentrations between 10 ^-8 and 10 ^-5 M. Cytokine concentration in the supernatants was tested using ELISA kits. DEX induced a dose-dependent inhibition of IL-10 production by PBMC from adults whereas CBMC from newborns were mostly unaffected by the drug. DEX caused a dose-dependent inhibition of IL-12p40 secretion by cells of the three age groups, although to a different extent. Since IL-12 plays a critical role in the development of a protective immune response to fungal infection, it is conceivable that the inhibition of IL-12p40 secretion caused by DEX may contribute to the increased occurrence of fungal infections in preterms treated with this drug.

Original language	English
Pages (from-to)	262-266
Number of pages	5
Journal	Biology of the Neonate
Volume	80
Issue number	4
DOIs	https://doi.org/10.1159/000047154
State	Published - 2001

Keywords

Dexamethasone
IL-10
IL-12p40
Mononuclear cells
Newborns

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10.1159/000047154

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title = "Effect of dexamethasone on IL-10 and IL-12p40 production in newborns and adults",

abstract = "The effect of dexamethasone (DEX) on interleukin (IL)-10 and IL-12p40 production was examined in preterm newborns, term infants and was compared to that in adults. Mononuclear cells isolated from newborn cord blood (CBMC) and peripheral blood from adults (PBMC) were incubated with lipopolysaccharide in the absence or presence of DEX at concentrations between 10 -8 and 10 -5 M. Cytokine concentration in the supernatants was tested using ELISA kits. DEX induced a dose-dependent inhibition of IL-10 production by PBMC from adults whereas CBMC from newborns were mostly unaffected by the drug. DEX caused a dose-dependent inhibition of IL-12p40 secretion by cells of the three age groups, although to a different extent. Since IL-12 plays a critical role in the development of a protective immune response to fungal infection, it is conceivable that the inhibition of IL-12p40 secretion caused by DEX may contribute to the increased occurrence of fungal infections in preterms treated with this drug.",

keywords = "Dexamethasone, IL-10, IL-12p40, Mononuclear cells, Newborns",

author = "Hanna Bessler and S. Kagazanov and I. Punsky and L. Sirota",

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AU - Bessler, Hanna

AU - Kagazanov, S.

AU - Punsky, I.

AU - Sirota, L.

PY - 2001

Y1 - 2001

N2 - The effect of dexamethasone (DEX) on interleukin (IL)-10 and IL-12p40 production was examined in preterm newborns, term infants and was compared to that in adults. Mononuclear cells isolated from newborn cord blood (CBMC) and peripheral blood from adults (PBMC) were incubated with lipopolysaccharide in the absence or presence of DEX at concentrations between 10 -8 and 10 -5 M. Cytokine concentration in the supernatants was tested using ELISA kits. DEX induced a dose-dependent inhibition of IL-10 production by PBMC from adults whereas CBMC from newborns were mostly unaffected by the drug. DEX caused a dose-dependent inhibition of IL-12p40 secretion by cells of the three age groups, although to a different extent. Since IL-12 plays a critical role in the development of a protective immune response to fungal infection, it is conceivable that the inhibition of IL-12p40 secretion caused by DEX may contribute to the increased occurrence of fungal infections in preterms treated with this drug.

AB - The effect of dexamethasone (DEX) on interleukin (IL)-10 and IL-12p40 production was examined in preterm newborns, term infants and was compared to that in adults. Mononuclear cells isolated from newborn cord blood (CBMC) and peripheral blood from adults (PBMC) were incubated with lipopolysaccharide in the absence or presence of DEX at concentrations between 10 -8 and 10 -5 M. Cytokine concentration in the supernatants was tested using ELISA kits. DEX induced a dose-dependent inhibition of IL-10 production by PBMC from adults whereas CBMC from newborns were mostly unaffected by the drug. DEX caused a dose-dependent inhibition of IL-12p40 secretion by cells of the three age groups, although to a different extent. Since IL-12 plays a critical role in the development of a protective immune response to fungal infection, it is conceivable that the inhibition of IL-12p40 secretion caused by DEX may contribute to the increased occurrence of fungal infections in preterms treated with this drug.

KW - Dexamethasone

KW - IL-10

KW - IL-12p40

KW - Mononuclear cells

KW - Newborns

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VL - 80

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EP - 266

JO - Biology of the Neonate

JF - Biology of the Neonate

IS - 4

ER -

Effect of dexamethasone on IL-10 and IL-12p40 production in newborns and adults

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Keywords

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