Effect of Chinese herbal therapy on breast cancer adenocarcinoma cell lines

Y. Maimon, V. Karaush, N. Yaal-Hahoshen, R. Ben-Yosef, I. Ron, A. Vexler, S. Lev-Ari

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Despite the widespread use of medicinal herbs to prevent and treat many diseases, including cancer, there are insufficient scientific data on the safety and efficacy of the majority of herbal therapies. The aim of this study was to assess the effect of a unique Chinese herbal therapy (CHT) fromcontrolledmanufactured concentrated powders, on an in vitro model of breast cancer. Three breast adenocarcinoma cell lines (MDA-231, MDA-453, T47D) were exposed to CHT for 72 h. Cell viability was assessed by XTT (sodium 3′-[1-(phenylaminocarbonyl)-3, 4-tetra zolium]-bis(4-methoxy-6-nitro) benzene sulphonic acid hydrate) assay. Apoptosis and cell cycle stage were determined by fluorescence-activated cell sorting (FACS) analysis. CHT decreased cell survival in a dose-dependent manner in all tested cell lines. FACS analysis of treated and non-treated T47D cells demonstrated that the inhibitory effect of CHT was associated with an increase in apoptosis. A randomized clinical trial is currently underway to investigate CHT as supplementary therapy for breast cancer patients receiving chemotherapy.

Original languageEnglish
Pages (from-to)2033-2039
Number of pages7
JournalJournal of International Medical Research
Volume38
Issue number6
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Apoptosis
  • Breast cancer
  • Chinese herbs
  • Facs analysis
  • Mda-231
  • Mda-453
  • T47d

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