Effect of adherent cells on the regulation of BFU‐E in patients with myeloproliferative disease

Evelyne Shabbad, Aliza Cassel*, Paul Froom, Esther Aghai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The interaction between adherent cells and red cell progenitors from peripheral blood of patients with polycythemia vera (PV), essential thrombocytosis (ET), and healthy controls was studied. Various combinations of adherent and nonadherent cells were co‐cultured in a semisolid system. Adherent cells from controls, when added at low concentrations, stimulated BFU‐E proliferation, whereas high concentrations (40% of total cells in the culture) caused a significant decrease in the number of BFU‐E colonies in 6/8 PV patients, 4/4 ET patients, and 8/12 controls. On the other hand, low and high concentrations of adherent cells from both patients with PV and ET caused a significant increase in BFU‐E from either patients or controls. Moreover, adherent cells from these patients induced endogenous BFU‐E proliferation (independent of erythropoietin) in nonadherent cells of 12/12 normal controls. The results show that BFU‐E from patients with PV and ET are sensitive to suppression by normal adherent cells. On the other hand, adherent cells from these patients possess stimulatory activity on BFU‐E from peripheral blood at all concentrations and are devoid of the inhibitory activity. This suggests a possible defect in the functioning of adherent cells in PV and ET patients which may contribute to the abnormal regulation of hematopoiesis in these disorders.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalAmerican Journal of Hematology
Volume33
Issue number4
DOIs
StatePublished - Apr 1990
Externally publishedYes

Keywords

  • erythropoiesis
  • monocytes
  • polycythemia vera

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