Effect of 1,25-dihydroxyvitamin D3 on interleukin 1β actions and cell growth in human synovial fibroblast cultures

I. Yaron, F. A. Meyer, Y. Weisman, M. Yaron

Research output: Contribution to journalArticlepeer-review

Abstract

Objective. To investigate the effects of vitamin D3 [1,25-(OH)2D3] metabolites on interleukin 1β (IL-1β) stimulated secretory activities and on the proliferation of human synovial fibroblasts in culture. Methods. Dose dependent effects on IL-1β actions were determined in nonproliferating cultures containing 1% fetal calf serum (FCS) in the culture medium. Production of prostaglandin E (PGE), collagenase and hyaluronic acid (HA) was measured respectively by radioimmunoassay, enzymatic degradation of radiolabelled collagen gels after collagenase activation and 14C- glucosamine incorporation. Effects on cell growth in 10% FCS were monitored colorimetrically, by staining cells with crystal violet. Results. 1,25- (OH)2D3 inhibited the effects of IL-1β on PGE production by up to 90%, with half maximal inhibition at 2.0 x 10-10 M. Inhibitory effects on stimulated collagenase and HA production and cell growth were also found but were less marked. At 10-7 M 1,25-(OH)2D3 inhibition was 50, 21 and 50%, respectively, 24,25-dihydroxyvitamin D3 was a less potent inhibitor than 1,25-(OH)2D3. Neither metabolite influenced IL-1β effects on PGE or sulfated glycosaminoglycan production in human articular cartilage in tissue culture. Conclusion. Our results suggest that the active metabolites of vitamin D3 may modulate the behavior of synovial fibroblasts in articular inflammatory processes.

Original languageEnglish
Pages (from-to)1527-1532
Number of pages6
JournalJournal of Rheumatology
Volume20
Issue number9
StatePublished - 1993
Externally publishedYes

Keywords

  • 1,25-(OH)D
  • CELL GROWTH
  • COLLAGENASE
  • PROSTAGLANDIN E
  • RHEUMATOID ARTHRITIS
  • SYNOVIAL FIBROBLASTS

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