TY - JOUR
T1 - Effect of α‐chlorohydrin on metabolism and testosterone secretion by rat testicular interstitial cells
AU - Paz, Gedalia
AU - Carmon, Ariela
AU - Homonnai, Zwi T.
PY - 1985/4
Y1 - 1985/4
N2 - The direct effect of α‐chlorochydrin (α‐CH) on basic metabolism (glucose utilitzation and oxygen consumption) and testosterone secretion by isolated rat interstitial cells (I‐cells) has been studied. In the range of concentrations between 5 and 100 μl/ml, only the highest doses of α‐CH decreased cell vitality and their histochemical stain for 3β‐HSD. Oxygen consumption of I‐cells was depressed at all doses higher than 10 μl/ml and this effect was reversible only with doses lower than 50 μl/ml. glucose utilization by I‐cells was depressed significantly by α‐CH and this effect was particularly dramatic with doses higher than 50 μl/ml. α‐CH decreased testosterone secretion by I‐cells, with maximal effects at 100 μl/ml. I‐cells responded to hCG challenge by increasing testosterone secretion, and hCG prevented the toxic effect of α‐CH at the lowest dose (10 μl/ml) of α‐CH, but failed to overcome the effects of a high dose (100 μl/ml).
AB - The direct effect of α‐chlorochydrin (α‐CH) on basic metabolism (glucose utilitzation and oxygen consumption) and testosterone secretion by isolated rat interstitial cells (I‐cells) has been studied. In the range of concentrations between 5 and 100 μl/ml, only the highest doses of α‐CH decreased cell vitality and their histochemical stain for 3β‐HSD. Oxygen consumption of I‐cells was depressed at all doses higher than 10 μl/ml and this effect was reversible only with doses lower than 50 μl/ml. glucose utilization by I‐cells was depressed significantly by α‐CH and this effect was particularly dramatic with doses higher than 50 μl/ml. α‐CH decreased testosterone secretion by I‐cells, with maximal effects at 100 μl/ml. I‐cells responded to hCG challenge by increasing testosterone secretion, and hCG prevented the toxic effect of α‐CH at the lowest dose (10 μl/ml) of α‐CH, but failed to overcome the effects of a high dose (100 μl/ml).
KW - 3β‐HSD
KW - interstitial cells
KW - testosterone
KW - α‐chlorohydrin
UR - http://www.scopus.com/inward/record.url?scp=0021790181&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2605.1985.tb00827.x
DO - 10.1111/j.1365-2605.1985.tb00827.x
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C2 - 3860478
AN - SCOPUS:0021790181
SN - 0105-6263
VL - 8
SP - 139
EP - 146
JO - International Journal of Andrology
JF - International Journal of Andrology
IS - 2
ER -