Dynamic three-wavelength imaging and volumetry of flowing cells with doubled field of view by six-pack holography

Simcha K. Mirsky; Natan T. Shaked

doi:10.1007/s00340-022-07812-z

Dynamic three-wavelength imaging and volumetry of flowing cells with doubled field of view by six-pack holography

Simcha K. Mirsky, Natan T. Shaked^*

^*Corresponding author for this work

Department of Bio-Medical Engineering

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We present a new six-pack holography (6PH) system for simultaneous acquisition of six off-axis holograms in a single exposure from two fields of view and three wavelengths. The system is based on an external 6PH module, placed at the output of an inverted microscope, presenting a significantly more elegant solution compared to previous 6PH systems, which were based on cumbersome Mach–Zehnder interferometers built around the sample. This is also the first time in which six-pack holography is utilized for multi-wavelength imaging. We demonstrate using the new system to acquire volumetric data of flowing erythrocytes with twice the field of view of previous multi-wavelength holographic techniques. This volumetric data includes mean corpuscular volume, mean corpuscular hemoglobin (Hb) concentration, mean corpuscular Hb, and sphericity.

Original language	English
Article number	92
Journal	Applied Physics B: Lasers and Optics
Volume	128
Issue number	5
DOIs	https://doi.org/10.1007/s00340-022-07812-z
State	Published - May 2022

Funding

Funders	Funder number
Horizon 2020 Framework Programme
H2020 European Research Council	678316

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10.1007/s00340-022-07812-z

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title = "Dynamic three-wavelength imaging and volumetry of flowing cells with doubled field of view by six-pack holography",

abstract = "We present a new six-pack holography (6PH) system for simultaneous acquisition of six off-axis holograms in a single exposure from two fields of view and three wavelengths. The system is based on an external 6PH module, placed at the output of an inverted microscope, presenting a significantly more elegant solution compared to previous 6PH systems, which were based on cumbersome Mach–Zehnder interferometers built around the sample. This is also the first time in which six-pack holography is utilized for multi-wavelength imaging. We demonstrate using the new system to acquire volumetric data of flowing erythrocytes with twice the field of view of previous multi-wavelength holographic techniques. This volumetric data includes mean corpuscular volume, mean corpuscular hemoglobin (Hb) concentration, mean corpuscular Hb, and sphericity.",

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Dynamic three-wavelength imaging and volumetry of flowing cells with doubled field of view by six-pack holography

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