Dual role of FtsH in regulating lipopolysaccharide biosynthesis in Escherichia coli

Chen Katz, Eliora Z. Ron

Research output: Contribution to journalArticlepeer-review

Abstract

In Escherichia coli, FtsH (HflB) is a membrane-bound, ATP-dependent metalloendoprotease belonging to the AAA family (ATPases associated with diverse cellular activities). FtsH has a limited spectrum of known substrates, including the transcriptional activator σ32. FtsH is the only known E. coli protease that is essential, as it regulates the concentration of LpxC, which carries out the first committed step in the synthesis of lipid A. Here we identify a new FtsH substrate - 3-deoxy-D-manno-octulosonate (KDO) transferase - which carries out the attachment of two KDO residues to the lipid A precursor (lipid IVA) to form the minimal essential structure of the lipopolysaccharide (LPS) (KDO2-lipid A). Thus, FtsH regulates the concentration of the lipid moiety of LPS (lipid A) as well as the sugar moiety (KDO-based core oligosaccharides), ensuring a balanced synthesis of LPS.

Original languageEnglish
Pages (from-to)7117-7122
Number of pages6
JournalJournal of Bacteriology
Volume190
Issue number21
DOIs
StatePublished - Nov 2008

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