Dual effects of opioids on transmitter release, intracellular calcium and calcium uptake in SK-N-SH cell line

Y. Sarne; M. Gafni; A. Fields; O. Keren

doi:10.1016/0167-0115(94)90485-5

Dual effects of opioids on transmitter release, intracellular calcium and calcium uptake in SK-N-SH cell line

Y. Sarne^*, M. Gafni, A. Fields, O. Keren

^*Corresponding author for this work

Physiology Pharmacology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The effect of opiates on KCl-induced neurotransmitter release (preloaded [³H] norepinephrine), intracellular calcium level (fura 2) and calcium influx (⁴⁵Ca⁺⁺ uptake) was measured in the human neuroblastoma SK-N-SH. Opiates exerted either inhibition or augmentation of transmitter release, both effects were blocked by naloxone. Similarly, opiates either reduced or potentiated KCl-induced elevation in cytosolic [Ca⁺⁺], with neighboring cells responding oppositely. The stimulatory effect of opiates on cytosolic calcium was dependent on the presence of extracellular calcium ions. Opiates stimulated calcium uptake by SK-N-SH cells. This stimulation was blocked by naloxone but was not prevented by pretreating the cells with pertusis toxin.

Original language	English
Pages (from-to)	253-254
Number of pages	2
Journal	Regulatory Peptides
Volume	54
Issue number	1
DOIs	https://doi.org/10.1016/0167-0115(94)90485-5
State	Published - 10 Nov 1994

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abstract = "The effect of opiates on KCl-induced neurotransmitter release (preloaded [3H] norepinephrine), intracellular calcium level (fura 2) and calcium influx (45Ca++ uptake) was measured in the human neuroblastoma SK-N-SH. Opiates exerted either inhibition or augmentation of transmitter release, both effects were blocked by naloxone. Similarly, opiates either reduced or potentiated KCl-induced elevation in cytosolic [Ca++], with neighboring cells responding oppositely. The stimulatory effect of opiates on cytosolic calcium was dependent on the presence of extracellular calcium ions. Opiates stimulated calcium uptake by SK-N-SH cells. This stimulation was blocked by naloxone but was not prevented by pretreating the cells with pertusis toxin.",

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AU - Keren, O.

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AB - The effect of opiates on KCl-induced neurotransmitter release (preloaded [3H] norepinephrine), intracellular calcium level (fura 2) and calcium influx (45Ca++ uptake) was measured in the human neuroblastoma SK-N-SH. Opiates exerted either inhibition or augmentation of transmitter release, both effects were blocked by naloxone. Similarly, opiates either reduced or potentiated KCl-induced elevation in cytosolic [Ca++], with neighboring cells responding oppositely. The stimulatory effect of opiates on cytosolic calcium was dependent on the presence of extracellular calcium ions. Opiates stimulated calcium uptake by SK-N-SH cells. This stimulation was blocked by naloxone but was not prevented by pretreating the cells with pertusis toxin.

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Dual effects of opioids on transmitter release, intracellular calcium and calcium uptake in SK-N-SH cell line

Abstract

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