Downregulation of microglial activation by achillolide A

Anat Elmann, Alona Telerman, Sharon Mordechay, Hilla Erlank, Miriam Rindner, Yoel Kashman, Rivka Ofir

Research output: Contribution to journalArticlepeer-review

Abstract

Chronic inflammation has been implicated in the pathogenesis of various neurodegenerative diseases. During the neuroinflammatory process, microglial cells release neurotoxic and proinflammatory mediators. In the present study, using activity-guided fractionation, we have purified an anti-inflammatory compound determined by spectroscopic methods to be a sesquiterpene lactone named achillolide A from Achillea fragrantissima (Forsk.) Sch. Bip. In primary cultures of lipopolysaccharide-activated microglial cells, achillolide A inhibited the lipopolysaccharide-induced levels of proinflammatory and toxic mediators including glutamate, nitric oxide, matrix metalloproteinase-9, cyclooxygenase-2, induced nitric oxide synthase, interleukin-1β, and tumor necrosis factor-α. Achillolide A also exhibited an antioxidant capacity, as was shown in a cell free system as well as by its ability to reduce intracellular reactive oxygen species levels in microglial cells. Thus, achillolide A might have therapeutic potential for treatment of neurodegenerative diseases and deserves further studies.

Original languageEnglish
Pages (from-to)215-221
Number of pages7
JournalPlanta Medica
Volume81
Issue number3
DOIs
StatePublished - Feb 2015

Keywords

  • Achillea fragrantissima
  • Asteraceae
  • achillolide A
  • inflammation
  • microglial cells
  • neurodegenerative diseases

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