Down-regulation of cardiac erythropoietin receptor and its downstream activated signal transducer phospho-STAT-5 in a rat model of chronic kidney disease

Einat Hertzberg-Bigelman, Rami Barashi, Ran Levy, Lena Cohen, Jeremy Ben-Shoshan, Gad Keren, Michal Entin-Meer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Chronic kidney disease (CKD) is often accompanied by impairment of cardiac function that may lead to major cardiac events. Erythropoietin (EPO), a kidney-produced protein, was shown to be beneficial to heart function. It was suggested that reduced EPO secretion in CKD may play a role in the initiation of heart damage. Objectives: To investigate molecular changes in the EPO/ erythropoietin receptor (EPO-R) axis in rat cardiomyocytes using a rat model for CKD. Methods: We established a rat model for CKD by kidney resection. Cardiac tissue sections were stained with Masson’s trichrome to assess interstitial fibrosis indicating cardiac damage. To evaluate changes in the EPO/EPO-R signaling cascade in the myocardium we measured cardiac EPO and EPO-R as well as the phosphorylation levels of STAT-5, a downstream element in this cascade. Results: At 11 weeks after resection, animals presented severe renal failure reflected by reduced creatinine clearance, elevated blood urea nitrogen and presence of anemia. Histological analysis revealed enhanced fibrosis in cardiac sections of CKD animals compared to the sham controls. Parallel to these changes, we found that although cardiac EPO levels were similar in both groups, the expression of EPO-R and the activated form of its downstream protein STAT-5 were significantly lower in CKD animals. Conclusions: CKD results in molecular changes in the EPO/EPO-R axis. These changes may play a role in early cardiac damage observed in the cardiorenal syndrome.

Original languageEnglish
Pages (from-to)326-330
Number of pages5
JournalIsrael Medical Association Journal
Volume18
Issue number6
StatePublished - Jun 2016

Keywords

  • Cardiac interstitial fibrosis
  • Chronic kidney disease (CKD)
  • Erythropoietin (EPO)
  • Erythropoietin receptor (EPO-R)
  • Phospho-STAT-5

Fingerprint

Dive into the research topics of 'Down-regulation of cardiac erythropoietin receptor and its downstream activated signal transducer phospho-STAT-5 in a rat model of chronic kidney disease'. Together they form a unique fingerprint.

Cite this