Double immunolabeling by the RBM and the PLAP markers for identifying intratubular (in situ) germ cell neoplasia of the testis

Letizia Schreiber; Beatriz Lifschitz-Mercer; Gedalia Paz; Haim Yavetz; David J. Elliott; Krzysztof Kula; Jolanta Slowikowska-Hilczer; Batia Bar Shira Maymon

doi:10.1177/106689690301100104

Double immunolabeling by the RBM and the PLAP markers for identifying intratubular (in situ) germ cell neoplasia of the testis

Letizia Schreiber, Beatriz Lifschitz-Mercer, Gedalia Paz, Haim Yavetz, David J. Elliott, Krzysztof Kula, Jolanta Slowikowska-Hilczer, Batia Bar Shira Maymon^*

^*Corresponding author for this work

Clinical Departments

Research output: Contribution to journal › Article › peer-review

Abstract

Identification of intratubular germ cell neoplasia (carcinoma in situ, CIS) of the testis is a diagnostic challenge, and markers are sorely needed to assist in accurately identifying the lesion. RNA-binding motif (RBM) protein, encoded by the Y chromosome, is expressed exclusively and consistently in differentiated male germ cells, while it is absent in neoplastic germ cells. Another immunohistochemical marker, placental alkaline phosphatase (PLAP), is commonly used for the detection of undifferentiated germ cells. The current study demonstrates that simultaneous use of the immunohistochemical markers, RBM and PLAP, by double immunolabeling enhances the accuracy of diagnosing CIS, a preinvasive testicular neoplasm.

Original language	English
Pages (from-to)	17-20
Number of pages	4
Journal	International Journal of Surgical Pathology
Volume	11
Issue number	1
DOIs	https://doi.org/10.1177/106689690301100104
State	Published - Jan 2003

Keywords

Carcinoma in situ
Immunohistochemistry
Intratubular germ cell neoplasia
Placental alkaline phosphatase (PLAP)
RNA-binding motif (RBM)
Testicular biopsies

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10.1177/106689690301100104

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title = "Double immunolabeling by the RBM and the PLAP markers for identifying intratubular (in situ) germ cell neoplasia of the testis",

abstract = "Identification of intratubular germ cell neoplasia (carcinoma in situ, CIS) of the testis is a diagnostic challenge, and markers are sorely needed to assist in accurately identifying the lesion. RNA-binding motif (RBM) protein, encoded by the Y chromosome, is expressed exclusively and consistently in differentiated male germ cells, while it is absent in neoplastic germ cells. Another immunohistochemical marker, placental alkaline phosphatase (PLAP), is commonly used for the detection of undifferentiated germ cells. The current study demonstrates that simultaneous use of the immunohistochemical markers, RBM and PLAP, by double immunolabeling enhances the accuracy of diagnosing CIS, a preinvasive testicular neoplasm.",

keywords = "Carcinoma in situ, Immunohistochemistry, Intratubular germ cell neoplasia, Placental alkaline phosphatase (PLAP), RNA-binding motif (RBM), Testicular biopsies",

author = "Letizia Schreiber and Beatriz Lifschitz-Mercer and Gedalia Paz and Haim Yavetz and Elliott, {David J.} and Krzysztof Kula and Jolanta Slowikowska-Hilczer and Maymon, {Batia Bar Shira}",

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month = jan,

doi = "10.1177/106689690301100104",

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AU - Schreiber, Letizia

AU - Lifschitz-Mercer, Beatriz

AU - Paz, Gedalia

AU - Yavetz, Haim

AU - Elliott, David J.

AU - Kula, Krzysztof

AU - Slowikowska-Hilczer, Jolanta

AU - Maymon, Batia Bar Shira

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AB - Identification of intratubular germ cell neoplasia (carcinoma in situ, CIS) of the testis is a diagnostic challenge, and markers are sorely needed to assist in accurately identifying the lesion. RNA-binding motif (RBM) protein, encoded by the Y chromosome, is expressed exclusively and consistently in differentiated male germ cells, while it is absent in neoplastic germ cells. Another immunohistochemical marker, placental alkaline phosphatase (PLAP), is commonly used for the detection of undifferentiated germ cells. The current study demonstrates that simultaneous use of the immunohistochemical markers, RBM and PLAP, by double immunolabeling enhances the accuracy of diagnosing CIS, a preinvasive testicular neoplasm.

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KW - Immunohistochemistry

KW - Intratubular germ cell neoplasia

KW - Placental alkaline phosphatase (PLAP)

KW - RNA-binding motif (RBM)

KW - Testicular biopsies

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Double immunolabeling by the RBM and the PLAP markers for identifying intratubular (in situ) germ cell neoplasia of the testis

Abstract

Keywords

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