TY - JOUR
T1 - Double immunolabeling by the RBM and the PLAP markers for identifying intratubular (in situ) germ cell neoplasia of the testis
AU - Schreiber, Letizia
AU - Lifschitz-Mercer, Beatriz
AU - Paz, Gedalia
AU - Yavetz, Haim
AU - Elliott, David J.
AU - Kula, Krzysztof
AU - Slowikowska-Hilczer, Jolanta
AU - Maymon, Batia Bar Shira
PY - 2003/1
Y1 - 2003/1
N2 - Identification of intratubular germ cell neoplasia (carcinoma in situ, CIS) of the testis is a diagnostic challenge, and markers are sorely needed to assist in accurately identifying the lesion. RNA-binding motif (RBM) protein, encoded by the Y chromosome, is expressed exclusively and consistently in differentiated male germ cells, while it is absent in neoplastic germ cells. Another immunohistochemical marker, placental alkaline phosphatase (PLAP), is commonly used for the detection of undifferentiated germ cells. The current study demonstrates that simultaneous use of the immunohistochemical markers, RBM and PLAP, by double immunolabeling enhances the accuracy of diagnosing CIS, a preinvasive testicular neoplasm.
AB - Identification of intratubular germ cell neoplasia (carcinoma in situ, CIS) of the testis is a diagnostic challenge, and markers are sorely needed to assist in accurately identifying the lesion. RNA-binding motif (RBM) protein, encoded by the Y chromosome, is expressed exclusively and consistently in differentiated male germ cells, while it is absent in neoplastic germ cells. Another immunohistochemical marker, placental alkaline phosphatase (PLAP), is commonly used for the detection of undifferentiated germ cells. The current study demonstrates that simultaneous use of the immunohistochemical markers, RBM and PLAP, by double immunolabeling enhances the accuracy of diagnosing CIS, a preinvasive testicular neoplasm.
KW - Carcinoma in situ
KW - Immunohistochemistry
KW - Intratubular germ cell neoplasia
KW - Placental alkaline phosphatase (PLAP)
KW - RNA-binding motif (RBM)
KW - Testicular biopsies
UR - http://www.scopus.com/inward/record.url?scp=0037286295&partnerID=8YFLogxK
U2 - 10.1177/106689690301100104
DO - 10.1177/106689690301100104
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AN - SCOPUS:0037286295
SN - 1066-8969
VL - 11
SP - 17
EP - 20
JO - International Journal of Surgical Pathology
JF - International Journal of Surgical Pathology
IS - 1
ER -